Department of Cadres Medical Care, The Second Hospital of Shandong University, Jinan, Shandong, China (mainland).
Analysis and Testing Center, Shandong University of Technology, Zibo, Shandong, China (mainland).
Med Sci Monit. 2019 Nov 10;25:8465-8471. doi: 10.12659/MSM.917823.
BACKGROUND The present study was designed to investigate the effect of wogonin on Caov-3 and A2780 ovary cancer cell proliferation and the mechanisms involved. MATERIAL AND METHODS Cell viability changes and apoptosis induction by wogonin were assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenytetrazolium bromide) assay and fluorescence microscopy. Morphological examination of cells was performed using transmission electron microscopy. RESULTS Wogonin exhibited inhibitory effect on Caov-3 and A2780 cancer cell proliferation in a concentration-based manner. Caov-3 and A2780 cell proliferation was reduced to 18% and 21%, respectively on treatment with 200 μM concentration of wogonin. Treatment with wogonin significantly enhanced the percentage of A2780 cells showing apoptosis. The nuclear membrane degradation and condensation of chromatin material was evident in A2780 cells on treatment with wogonin. Treatment of A2780 cells with wogonin suppressed the migration potential significantly. The proportion of A2780 cells in G1/G0 phase was markedly raised on exposure to wogonin for 48 hours. CONCLUSIONS In summary, this study demonstrated that wogonin acts as an ovary cancer cell proliferation inhibiting agent through activation of apoptosis. Wogonin, therefore, can be investigated further for the development of ovary cancer treatment.
本研究旨在探讨白杨素对 Caov-3 和 A2780 卵巢癌细胞增殖的影响及其作用机制。
通过 MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）法和荧光显微镜评估白杨素对细胞活力变化和凋亡的诱导作用。使用透射电子显微镜对细胞形态进行检查。
白杨素呈浓度依赖性地抑制 Caov-3 和 A2780 癌细胞的增殖。用 200 μM 浓度的白杨素处理后，Caov-3 和 A2780 细胞的增殖分别减少到 18%和 21%。用白杨素处理可显著增加表现出凋亡的 A2780 细胞的百分比。用白杨素处理 A2780 细胞时，核膜降解和染色质物质的浓缩明显。用白杨素处理 A2780 细胞可显著抑制其迁移潜力。暴露于白杨素 48 小时后，A2780 细胞中 G1/G0 期的比例明显升高。
总之，本研究表明，白杨素通过激活细胞凋亡来抑制卵巢癌细胞的增殖。因此，白杨素可进一步研究用于卵巢癌的治疗。
