[细胞周期蛋白与急性白血病预后的关系]
[Relationship between cyclins and prognosis of acute leukemia].
作者信息
Wu Sui-Jing, Du Xin, Chen Yun-Xian, Jiang Wen-Ling, Zhong Li-Ye, Lin Wei, Huang Zi-Lun
机构信息
Department of Hematology, Guangdong Provincial People's Hospital, Guangzhou, Guangdong, 510080, PR China.
出版信息
Ai Zheng. 2003 Aug;22(8):852-5.
BACKGROUND & OBJECTIVE: Cell proliferation and differentiation are directed by cell cycle mechanism. When tumor cells proliferate abnormally, cyclins, which are positive agents of cell cycle, may be expressed abnormally at the same time. Now many references proved that cyclins are highly expressed in solid tumors. This study was designed to investigate the relationship between expression of cyclins and prognosis of acute leukemia.
METHODS
Sixty-eight cases of acute leukemia were enrolled. Reverse transcription polymerase chain reaction (RT-PCR) was performed on tumor samples to examine the expression of cyclin A, cyclin D, cyclin E. All samples were divided into three groups: acute leukemia in complete remission (12 cases), newly diagnosed acute leukemia (16 cases) and refractory leukemia (40 case). Samples of benign hemopoietic patients were used as normal control (15 cases).
RESULTS
All the 15 cases in the control group were negative of cyclin mRNA. No difference of cyclin A mRNA expression was shown between control group and the three experiment groups (P >0.05). But expression of cyclin D and cyclin E mRNA was significantly different between them (P< 0.01). There was no difference of expression of cyclin D and cyclin E mRNA among CR patients with acute leukemia(P >0.05), while the expression of cyclin D mRNA is significantly higher than that of cyclin E in refractory leukemia group. Furthermore the expression of cyclin D mRNA in recurrent refractory leukemia patients is significantly higher than that newly diagnosed cases (P< 0.05). All cyclins mRNA positive cases were divided into single cyclin gene expressed group and multiple cyclins gene expressed group. The positive rates of them were counted. No difference was found between complete remission group and refractory leukemia group (P >0.05).
CONCLUSION
Cyclin D may act as a prognostic marker for acute leukemia. The amount of cyclins expressed cannot be used as a prognostic factor for acute leukemia.
背景与目的
细胞增殖和分化受细胞周期机制调控。当肿瘤细胞异常增殖时，作为细胞周期正向调节因子的细胞周期蛋白可能同时出现异常表达。目前诸多文献证实细胞周期蛋白在实体瘤中高表达。本研究旨在探讨细胞周期蛋白表达与急性白血病预后的关系。
方法
纳入68例急性白血病患者。采用逆转录聚合酶链反应（RT-PCR）检测肿瘤样本中细胞周期蛋白A、细胞周期蛋白D、细胞周期蛋白E的表达。所有样本分为三组：急性白血病完全缓解组（12例）、初诊急性白血病组（16例）和难治性白血病组（40例）。选取良性造血患者样本作为正常对照（15例）。
结果
对照组15例样本细胞周期蛋白mRNA均为阴性。对照组与三个实验组细胞周期蛋白A mRNA表达无差异（P>0.05）。但细胞周期蛋白D和细胞周期蛋白E mRNA表达在它们之间有显著差异（P<0.01）。急性白血病完全缓解患者中细胞周期蛋白D和细胞周期蛋白E mRNA表达无差异（P>0.05），而难治性白血病组中细胞周期蛋白D mRNA表达显著高于细胞周期蛋白E。此外，复发难治性白血病患者细胞周期蛋白D mRNA表达显著高于初诊病例（P<0.05）。将所有细胞周期蛋白mRNA阳性病例分为单周期蛋白基因表达组和多周期蛋白基因表达组，统计其阳性率。完全缓解组与难治性白血病组之间无差异（P>0.05）。
结论
细胞周期蛋白D可能作为急性白血病的预后标志物。细胞周期蛋白的表达量不能作为急性白血病的预后因素。
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