Avila Andréa Rodrigues, Dallagiovanna Bruno, Yamada-Ogatta Sueli Fumie, Monteiro-Góes Viviane, Fragoso Stênio Perdigão, Krieger Marco Aurélio, Goldenberg Samuel
Instituto de Biologia Molecular do Paraná, Rua Professor Algacyr Munhoz Mader, 3775, 81350-010, Curitiba, PR, Brasil.
Genet Mol Res. 2003 Mar 31;2(1):159-68.
The process of Trypanosoma cruzi metacyclogenesis involves the transformation of noninfective epimastigotes into metacyclic trypomastigotes, which are the pathogenic form. The analysis of stage-specific genes during T. cruzi metacyclogenesis may provide insight into the mechanisms involved in the regulation of gene expression in trypanosomatids. It may also improve the understanding of the mechanisms responsible for the pathology of Chagas disease, and could lead to the identification of new targets for chemotherapy of this disease. We have demonstrated that during metacyclogenesis the expression of several genes is controlled at the translational level by an alternative regulatory mechanism. This mechanism may involve the mobilization of mRNA to the translation machinery. We have been using self-made T. cruzi microarrays to investigate the role of polysomal mobilization in modulating gene expression during metacyclogenesis.
克氏锥虫循环后期发育过程涉及无感染性的上鞭毛体转化为循环后期锥鞭毛体，后者是致病形式。对克氏锥虫循环后期发育过程中阶段特异性基因的分析，可能有助于深入了解锥虫中基因表达调控的机制。这也可能增进对恰加斯病病理机制的理解，并有助于识别该疾病化疗的新靶点。我们已经证明，在循环后期发育过程中，几个基因的表达是通过一种替代调控机制在翻译水平上受到控制的。这种机制可能涉及将信使核糖核酸转运到翻译机制中。我们一直在使用自制的克氏锥虫微阵列来研究多核糖体转运在调节循环后期发育过程中基因表达方面的作用。
