De-Simone Salvatore G, Bourguignon Saulo C, Gonçalves Priscila S, Lechuga Guilherme C, Provance David W
Center for Technological Development in Health (CDTS), FIOCRUZ, National Institute of Science and Technology for Innovation in Neglected Populations Diseases (INCT-IDPN), Rio de Janeiro 21040-900, RJ, Brazil.
Epidemiology and Molecular Systematic Laboratory, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil.
Pathogens. 2022 Feb 19;11(2):268. doi: 10.3390/pathogens11020268.
Intracellular parasites such as need to acquire valuable carbon sources from the host cell to replicate. Here, we investigated the energetic metabolism of during metacyclogenesis through the determination of enzymatic activities and quantification by HPLC of glycolytic and Krebs cycle short-chain carboxylic acids. Altered concentrations in pyruvate, acetate, succinate, and glycerate were measured during the growth of epimastigote in the complex medium BHI and their differentiation to trypomastigotes in the chemically defined medium, TAU3AAG. These alterations should represent significant differential metabolic modifications utilized by either form to generate energy. This paper is the first work dealing with the intracellular organic acid concentration measurement in parasites. Although it confirms the previous assumption of the importance of carbohydrate metabolism, it yields an essential improvement in metabolism knowledge.
诸如 这样的细胞内寄生虫需要从宿主细胞获取有价值的碳源以进行复制。在此，我们通过测定酶活性以及用高效液相色谱法对糖酵解和三羧酸循环的短链羧酸进行定量，研究了 在变循环前期的能量代谢。在复杂培养基BHI中，对 型鞭毛虫生长过程中以及它们在化学成分明确的培养基TAU3AAG中分化为锥鞭毛虫过程中的丙酮酸、乙酸、琥珀酸和甘油酸浓度变化进行了测量。这些变化应代表了两种形态用于产生能量的显著不同的代谢修饰。本文是第一篇涉及 寄生虫细胞内有机酸浓度测量的研究。尽管它证实了先前关于碳水化合物代谢重要性的假设，但它在 代谢知识方面有了实质性的改进。
