Bosetti M, Vernè E, Brovarone C Vitale, Moisescu C, Sabbatini M, Cannas M
Department of Medical Sciences, Human Anatomy, University Eastern Piedmont, A. Avogadro, Via Solaroli 17, 28100 Novara, Italy.
J Biomed Mater Res A. 2003 Sep 1;66(3):615-21. doi: 10.1002/jbm.a.10030.
The results of a surface analysis performed on a fluoroapatite-based glass ceramic (SAF) also coating a full-density alpha-alumina substrate (SAF-alumina coating) are presented. These two materials have also been evaluated after soaking in simulated body fluid to understand their ability to induce hydroxyapatite growth on them. Aiming to understand the fluoroapatite glass-ceramic interaction with some plasma proteins, in the second part of this study, fibronectin, albumin, immunoglobulin G, IgA, and complement factor C3c SAF binding have been evaluated; surface activity on complement activation has also been quantified. SAF-alumina coating provides good sites for the nucleation and growth of an apatite layer, equivalent to the mineral component of bone and binds preferentially plasma fibronectin, which is well known to enhance cell adhesion and spreading. Moreover, SAF-alumina coating reduces alumina complement activation directly or via reduced IgA binding. Alumina was shown to bind the same C3 fragments as Zymosan, used as complement activating control, and to induce increased levels of serum soluble iC3b and Bb. A mechanical resistant material with enhanced bioactivity, bone integration, and reduced inflammatory potential respect to alumina has been obtained.
本文展示了对一种基于氟磷灰石的玻璃陶瓷（SAF）在全密度α-氧化铝基底上涂层（SAF-氧化铝涂层）进行表面分析的结果。这两种材料在模拟体液中浸泡后也进行了评估，以了解它们诱导羟基磷灰石在其上生长的能力。为了了解氟磷灰石玻璃陶瓷与某些血浆蛋白的相互作用，在本研究的第二部分，评估了纤连蛋白、白蛋白、免疫球蛋白G、IgA和补体因子C3c与SAF的结合；还对补体激活的表面活性进行了定量。SAF-氧化铝涂层为磷灰石层的成核和生长提供了良好的位点，等同于骨的矿物质成分，并优先结合血浆纤连蛋白，众所周知，纤连蛋白可增强细胞黏附和铺展。此外，SAF-氧化铝涂层直接或通过减少IgA结合来降低氧化铝补体激活。氧化铝被证明与用作补体激活对照的酵母聚糖结合相同的C3片段，并诱导血清可溶性iC3b和Bb水平升高。获得了一种相对于氧化铝具有增强的生物活性、骨整合能力和降低的炎症潜能的机械抗性材料。
