Agrawal Anurag A, Kurashige Nile S
Department of Botany, University of Toronto, 25 Willcocks Street, Toronto, Ontario M5S 3B2, Canada.
J Chem Ecol. 2003 Jun;29(6):1403-15. doi: 10.1023/a:1024265420375.
We experimentally reanalyzed the classic interaction between Pieris rapae, a specialist lepidopteran herbivore, and isothiocyanates (mustard oils) that are characteristic phytochemicals of the Brassicaceae. Previous investigations have suggested that P. rapae is unaffected by isothiocyanates. Using whole plants, root extracts, and a microencapsulated formulation of allyl isothiocyanate, we now show that isothiocyanates reduce herbivore survival and growth, and increase development time, each in a dose-dependent manner. Neither the substrate allyl glucosinolate, nor myrosinase, the enzyme that results in the breakdown of glucosinolates, negatively affected P. rapae. Thus, we present strong evidence for a role for isothiocyanates in plant resistance against the specialist herbivore P. rapae.
我们通过实验重新分析了菜粉蝶（一种专食性鳞翅目食草动物）与异硫氰酸盐（芥子油）之间的经典相互作用，异硫氰酸盐是十字花科植物特有的植物化学物质。先前的研究表明，菜粉蝶不受异硫氰酸盐的影响。我们现在使用整株植物、根提取物和烯丙基异硫氰酸盐的微胶囊制剂表明，异硫氰酸盐会降低食草动物的存活率和生长率，并延长发育时间，且每种影响均呈剂量依赖性。底物烯丙基硫代葡萄糖苷和导致硫代葡萄糖苷分解的酶——黑芥子酶，均未对菜粉蝶产生负面影响。因此，我们提供了有力证据，证明异硫氰酸盐在植物抵御专食性食草动物菜粉蝶方面发挥了作用。
