van Dam Nicole M, van der Meijden Ed, Verpoorte Robert
Institute of Evolutionary and Ecological Sciences, University of Leiden, P.O. Box 9516, 2300 RA, Leiden, The Netherlands.
Institute for Bio-Pharmaceutical Sciences, University of Leiden, P.O. Box 9502, 2300 RA, Leiden, The Netherlands.
Oecologia. 1993 Sep;95(3):425-430. doi: 10.1007/BF00320998.
In this paper we test three plant species for the inducibility of their alkaloid production. The plants were heavily damaged by cutting off 50% of their leaf surface using a pair of scissors. The cut-off leaf tips were used as controls for possible diurnal fluctuations. After 3, 6, 12, 24 and 48 h, respectively, the leaf bases of the damaged plants were harvested and the alkaloid concentration was measured. In Senecio jacobaea the pyrrolizidine alkaloid (Pa) concentration in damaged plants decreased within 6-12 h after damage. Within 24 h after damage the Pa concentration of Cynoglossum officinale doubled compared to control values. Indole alkaloid production in Catharanthus roseus was found not to be induced in this experiment. The responses are discussed in a functional context. We hypothesize that the nature of the response is not a feature of the type of secondary metabolite, but is related to whether the plants are damaged mainly by generalist or by specialist herbivores.
在本文中，我们测试了三种植物物种生物碱产生的可诱导性。使用剪刀剪掉50%的叶片表面，使这些植物受到严重损伤。剪下的叶尖用作可能的昼夜波动的对照。分别在3、6、12、24和48小时后，采集受损植物的叶基部并测量生物碱浓度。在千里光中，受损植物中吡咯里西啶生物碱（Pa）的浓度在受损后6 - 12小时内下降。受损后24小时内，药用琉璃草的Pa浓度与对照值相比增加了一倍。在本实验中发现长春花的吲哚生物碱产量未被诱导。在功能背景下讨论了这些反应。我们假设，反应的性质不是次生代谢物类型的特征，而是与植物主要是被广食性还是专食性食草动物损害有关。
