Hsu Feng-Liu, Chen Chun-Hsien, Yuan Cheng-Hui, Shiea Jentaie
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Anal Chem. 2003 May 15;75(10):2493-8. doi: 10.1021/ac0203746.
This study has developed two interfaces to connect small-size thin-layer chromatography (TLC) with electrospray ionization mass spectrometry (ES-MS) for the continuous analysis of organic mixtures. The interfaces are (1) two bound optical fibers inserted into the C18-bonded particles at the exit of a small TLC channel and (2) a small commercial TLC strip with a sharpened tip. A reservoir continuously supplied a makeup solution to the tip of the TLC channel. The high voltage required for electrospray ionization was introduced into the makeup solution or mobile phase through a Pt wire, and electrospray was generated at the tip of the bonded optical fibers and at the sharp end of the TLC strip. Since small-size TLC channels were used, the elution time was short and less than 0.2 microL of the sample solution and 200 microL of the eluting solvent were required. Organic mixtures were separated successfully and detected on-line using the TLC/ES-MS techniques.
本研究开发了两种接口，用于连接小型薄层色谱（TLC）和电喷雾电离质谱（ES-MS），以对有机混合物进行连续分析。这两种接口分别是：（1）两根插入小型TLC通道出口处C18键合颗粒中的束缚光纤；（2）一个带有尖锐尖端的小型商用TLC条带。一个储液器持续向TLC通道的尖端供应补充溶液。电喷雾电离所需的高电压通过一根铂丝引入补充溶液或流动相中，并在键合光纤的尖端和TLC条带的尖锐末端产生电喷雾。由于使用了小型TLC通道，洗脱时间较短，所需样品溶液少于0.2微升，洗脱溶剂为200微升。利用TLC/ES-MS技术成功分离并在线检测了有机混合物。
