Usami A, Abe S, Ide Y
Department of Anatomy, Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba-City, Chiba, Japan.
Anat Histol Embryol. 2003 Aug;32(4):244-8. doi: 10.1046/j.1439-0264.2003.00481.x.
Masticatory muscles that are derived from the branchial arches express different compositions of myosin heavy chain (MHC) isoforms during the transitional phase from suckling to mastication. To clarify the developmental changes of murine masseter muscle, the composition of MHC isoforms was examined using immunohistochemical staining and competitive reverse transcription PCR. We found that MHC1 was expressed transiently in the pre and post-natal stages. In the compositional change of isoforms, the embryonic type MHCp was expressed consistently, whereas the adult isoforms increased with the developmental process. In particular, a significant change was observed between embryonic days 14 and 16, a stage when murine facial development is conspicuous. This suggests that the development of murine masseter muscle is closely associated with facial development.
源自鳃弓的咀嚼肌在从哺乳到咀嚼的过渡阶段表达不同组成的肌球蛋白重链（MHC）亚型。为了阐明小鼠咬肌的发育变化，采用免疫组织化学染色和竞争性逆转录PCR检测了MHC亚型的组成。我们发现MHC1在出生前和出生后阶段短暂表达。在亚型的组成变化中，胚胎型MHCp持续表达，而成人亚型则随着发育过程增加。特别是在胚胎第14天到16天之间观察到显著变化，这是小鼠面部发育明显的阶段。这表明小鼠咬肌的发育与面部发育密切相关。
