Monemi M, Eriksson P O, Dubail I, Butler-Browne G S, Thornell L E
Department of Clinical Oral Physiology, Umeå University , Sweden.
FEBS Lett. 1996 May 13;386(1):87-90. doi: 10.1016/0014-5793(96)00402-4.
Biochemical, immunohistochemical and molecular biological methods were used to detect fetal myosin heavy chain (MyHC) in the human masseter of elderly and young subjects. Samples from the elderly subjects contained larger amounts of fetal MyHC than those of young adults. Only a very small amount of embryonic MyHC could be detected in both age groups. Embryonic and fetal MyHCs were never detected in the control adult orofacial, limb and trunk muscles. Polymerase chain reaction (PCR) analysis revealed the presence of fetal mRNA sequences in elderly and young masseter muscles. We conclude that fetal MyHC is present in the human masseter throughout the life span and that there is an increase in the relative amount of this protein with age.
采用生化、免疫组化和分子生物学方法检测老年人和年轻人咬肌中的胎儿肌球蛋白重链（MyHC）。老年受试者样本中所含胎儿MyHC的量比年轻成年人的多。在两个年龄组中均仅能检测到极少量的胚胎型MyHC。在对照的成年口腔面部、肢体和躯干肌肉中从未检测到胚胎型和胎儿型MyHC。聚合酶链反应（PCR）分析显示老年人和年轻人的咬肌中存在胎儿型mRNA序列。我们得出结论，胎儿型MyHC在人类咬肌中终生存在，并且该蛋白的相对含量随年龄增长而增加。
