Apostoli P, Mangili A, Carasi S, Manno M
Chair of Industrial Hygiene, Department of Experimental and Applied Medicine, University of Brescia, P.le Spedali Civili, 1, Brescia 25123, Italy.
Toxicol Lett. 2003 Sep 15;144(1):17-26. doi: 10.1016/s0378-4274(03)00226-1.
The polychlorinated biphenyls (PCBs) have been demonstrated to be inducers of hepatic microsomal enzymes and some of their effects such as hormonal imbalance, and alteration of lipid and porphyrin metabolism could be ascribed to this mechanism. For this reason, the urinary excretion of D-glucaric acid (DGA), an indirect indicator of enzymatic induction, was suggested as a biological marker of effect following exposure to PCBs. The aim of the present study was to investigate whether any inductive effects resulting from exposure to these compounds through ingestion of contaminated food could be detected early by measuring urinary DGA (U-DGA). U-DGA was measured in 73 subjects exposed to PCBs due to ingestion of PCB-contaminated food and levels ranged from 1.7 to 12.4 mmol/mol creatinine, with a mean value of 5.96 mmol/mol creatinine. These values were higher than those usually found in the general population. Sex and smoking habits did not affect U-DGA excretion, while age and alcohol intake were significantly correlated with U-DGA excretion, a finding in agreement with the results of other investigations. Neither total PCB blood concentration nor PCB chlorine content was significantly correlated with U-DGA excretion, and only the PCB 138 congener was weakly correlated with U-DGA levels. The results indicate that, for exposure to PCB resulting in blood concentrations up to 394 microg/l, no statistically significant effect of these persistent organochlorine compounds on human enzyme induction could be demonstrated, as measured by DGA urinary excretion.
多氯联苯（PCBs）已被证明是肝微粒体酶的诱导剂，其一些影响，如激素失衡以及脂质和卟啉代谢的改变，可能归因于这一机制。因此，尿中D-葡糖醛酸（DGA）的排泄作为酶诱导的间接指标，被提议作为接触多氯联苯后效应的生物标志物。本研究的目的是调查通过摄入受污染食物接触这些化合物所产生的任何诱导效应，是否可以通过测量尿DGA（U-DGA）早期检测到。对73名因摄入受多氯联苯污染食物而接触多氯联苯的受试者测量了U-DGA，其水平范围为1.7至12.4 mmol/mol肌酐，平均值为5.96 mmol/mol肌酐。这些值高于一般人群中通常发现的值。性别和吸烟习惯不影响U-DGA排泄，而年龄和酒精摄入量与U-DGA排泄显著相关，这一发现与其他调查结果一致。总多氯联苯血浓度和多氯联苯氯含量均与U-DGA排泄无显著相关性，只有多氯联苯138同系物与U-DGA水平呈弱相关。结果表明，对于接触多氯联苯导致血浓度高达394μg/l的情况，通过尿DGA排泄测量，这些持久性有机氯化合物对人体酶诱导没有统计学上的显著影响。
