Sandstad O, Osnes T, Skar V, Osnes M
Department of Internal Medicine, Ullevål Hospital, Oslo, Norway.
Scand J Clin Lab Invest. 1993 Jul;53(4):327-33. doi: 10.3109/00365519309086623.
D-glucaric acid, an end product of glucuronic acid metabolism, has been used as a marker substance for microsomal enzyme induction. In this study a convenient microtitre-plate based method for the quantification of urinary D-glucaric acid has been developed and validated. Mean urinary D-glucaric acid excretion in 20 health humans as measured by this method was 3.2 mumol glucaric acid mmol-1 creatinine, 95% confidence interval 3.0-3.4. Moderate alcohol consumption in 18 healthy volunteers did not significantly augment the urinary D-glucaric acid excretion. Occupational exposition to toluene in a printing plant was investigated. In spite of considerable intra- and inter-individual variability, a significant difference between exposed (3.5, 3.1-3.9) and non-exposed (2.6, 2.2-3.0) workers was observed, p < 0.025. We conclude that the measurement of D-glucaric acid can be utilized for biological screening of enzyme induction on a group basis.
D-葡糖二酸是葡糖醛酸代谢的终产物，已被用作微粒体酶诱导的标志物。在本研究中，已开发并验证了一种基于微量滴定板的便捷方法，用于定量测定尿中D-葡糖二酸。用该方法测得的20名健康人的尿中D-葡糖二酸平均排泄量为3.2 μmol葡糖二酸/ mmol肌酐，95%置信区间为3.0 - 3.4。18名健康志愿者适度饮酒并未显著增加尿中D-葡糖二酸的排泄量。对一家印刷厂工人的职业性甲苯暴露情况进行了调查。尽管个体内和个体间存在相当大的变异性，但观察到暴露组（3.5，3.1 - 3.9）和未暴露组（2.6，2.2 - 3.0）工人之间存在显著差异，p < 0.025。我们得出结论，D-葡糖二酸的测定可用于基于群体的酶诱导生物筛查。
