平阳霉素对人血管内皮细胞生长及细胞周期的影响

[Effect of pingyangmycin on the growth and cell cycle of human vascular endothelial cells].

作者信息

Kong Wei-dong, Li Yan-hao, Zeng Qing-le, Chen Yong, He Xiao-feng

机构信息

Department of Interventional Radiology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.

出版信息

Di Yi Jun Yi Da Xue Xue Bao. 2003 Aug;23(8):830-2, 836.

PMID:12919911

Abstract

OBJECTIVE

To study the effect of pingyangmycin (PYM, bleomycin A5) on the proliferation and cell cycle of the cultured ECV304 cells, a human umbilical vein endothelial cell (HUVEC) line.

METHODS

The growth inhibition of PYM on ECV304 cells was measured by MTT assay and the changes in the cell cycle by flow cytometry.

RESULTS

After 10 microg/ml PYM treatment of the cells for 24, 48, and 72 h, the inhibition rates were 44.7%, 59.7%, and 74.4% respectively, showing a dose- and time-dependent inhibitory effect, with the 50% inhibitory concentration (IC50) of PYM corresponding to treatment durations of 24, 48 and 72 h being 32.94, 2.56 and 0.75 microg/ml respectively. Flow cytometry showed that ECV304 cell cycle was arrested at G2-M phase after 24-hour treatment with 1 microg/ml PYM, with significant reduction in the cell ratio of S phase and increase of G2-M phase (P<0.01)

CONCLUSION

PYM can effectively inhibit the proliferation of ECV304 cells, the mechanism of which might involve the blocking of cell cycle.

摘要

目的

研究平阳霉素（PYM，博来霉素A5）对人脐静脉内皮细胞系ECV304细胞增殖及细胞周期的影响。

方法

采用MTT法检测PYM对ECV304细胞的生长抑制作用，并用流式细胞术检测细胞周期的变化。

结果

用10μg/ml PYM处理细胞24、48和72小时后，抑制率分别为44.7%、59.7%和74.4%，呈剂量和时间依赖性抑制作用，PYM在24、48和72小时处理时的50%抑制浓度（IC50）分别为32.94、2.56和0.75μg/ml。流式细胞术显示，用1μg/ml PYM处理24小时后，ECV304细胞周期停滞在G2-M期，S期细胞比例显著降低，G2-M期细胞比例增加（P<0.01）。