灵芝孢子对HepG2细胞增殖及生长周期的影响
[Effects of Ganoderma lucidum spores on HepG2 cells proliferation and growth cycle].
作者信息
Li Lin, Li Ting, Wang Xiao-Jing, Xu Jiang-Ping, Wang Shun-Guan
机构信息
Department of Pharmacology, School of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China.
出版信息
Zhong Yao Cai. 2008 Oct;31(10):1514-8.
OBJECTIVE
To observe the effects of Ganoderma lucidum Spores (GLS) on proliferation and growth cycle in the human hepatoma cell line (HepG2 cells), and study its possible mechanism of activities.
METHODS
The growth inhibition of GLS on HepG2 cells was detected by MTT assay. The DNA contents and the distribution of cell cycle were analyzed by flow cytometry.
RESULTS
The results of MTT assays showed that GLS could inhibit the HepG2 cells growth at a dose and time-dependent manner directly; the inhibition rate of GLS (2500 microg/ml) on HepG2 cells after 72 h was a maximum up 51.4%. The results of flow cytometry experiments showed that GLS (3 mg/ml) could reduce the G2 phase of HepG2 cells and a clear apoptosis peak would be observed when GLS was 6 mg/ml.
CONCLUSION
GLS has a direct inhibitory effect on tumor cell proliferation and its growth cycle, it can reduce the G2 phase; and high doses of GLS can also make tumor cells apoptosised.
目的
观察灵芝孢子粉（GLS）对人肝癌细胞系（HepG2细胞）增殖及生长周期的影响，并探讨其可能的作用机制。
方法
采用MTT法检测GLS对HepG2细胞的生长抑制作用。通过流式细胞术分析DNA含量及细胞周期分布。
结果
MTT法结果显示，GLS可直接以剂量和时间依赖性方式抑制HepG2细胞生长；72 h后，2500 μg/ml的GLS对HepG2细胞的抑制率最高可达51.4%。流式细胞术实验结果表明，3 mg/ml的GLS可使HepG2细胞的G2期减少，当GLS为6 mg/ml时可观察到明显的凋亡峰。
结论
GLS对肿瘤细胞增殖及其生长周期具有直接抑制作用，可使G2期减少；高剂量的GLS还可使肿瘤细胞凋亡。
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