Cai Hong-Wei, Yuyama Nana, Tamaki Hiroyuki, Yoshizawa Akira
Japan Grassland Farming and Forage Seed Association, Forage Crop Research Institute, 388-5, Higashiakada, Nishinasuno, Tochigi 329-2742, Japan.
Theor Appl Genet. 2003 Nov;107(8):1337-49. doi: 10.1007/s00122-003-1386-x. Epub 2003 Aug 15.
To develop simple sequence repeat (SSR) markers for the hexaploid forage grass timothy ( Phleum pratense L.), we used four SSR-enriched genomic libraries to isolate 1,331 SSR-containing clones. All four libraries contained a high percentage of perfect clones, ranging from 78.1% to 91.6%. From these clones, we developed 355 SSR markers when tested from 502 SSR primer pairs. Using all 355 SSR markers we tested one screening panel consisting of eight timothy clones to detect the level of polymorphism and identify a set of loci suitable for framework mapping. The SSR markers detected 90.4% polymorphism between the parents of a pseudo-testcross F(1) population. These SSR markers will provide an ideal marker system to assist with gene targeting, QTL (quantitative trait locus) mapping, and marker-assisted selection in timothy.
为开发六倍体饲用牧草梯牧草（Phleum pratense L.）的简单序列重复（SSR）标记，我们利用四个富含SSR的基因组文库分离出1331个含SSR的克隆。所有四个文库中完美克隆的比例都很高，范围在78.1%至91.6%之间。从这些克隆中，当用502对SSR引物对进行测试时，我们开发出了355个SSR标记。使用所有355个SSR标记，我们检测了一个由八个梯牧草克隆组成的筛选面板，以检测多态性水平并鉴定出一组适合构建框架图谱的位点。SSR标记在一个假测交F(1)群体的亲本间检测到90.4%的多态性。这些SSR标记将为梯牧草的基因靶向、QTL（数量性状位点）定位和标记辅助选择提供一个理想的标记系统。
