Bo Bin, Wang Chang-yong, Guo Xi-min
Tissue Engineering Research Center, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing, P. R. China, 100850.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2003 Jul;17(4):335-8.
To determine whether culture expanded bone marrow derived mesenchymal stem cells (MSCs) in combination with beta-tricalcium phosphate(beta-TCP) can repair critical cranial defects in New Zealand rabbits.
In group A(n = 20), MSCs from homogeneous rabbits were isolated and expanded in vitro and then implanted onto the pre-molded porous beta-TCP. The MSCs-beta-TCP complexes were implanted into rabbit critical cranial defects. In group B (n = 10), The defects were repaired with beta-TCP only. In group C(n = 4), the defects were left un-repaired. Samples were extracted 6 and 12 weeks after operation for histological, histochemical and immunohistochemical analysis.
In group A, bone-like tissue formation could be seen on the surface of the implants. Microscopic analysis demonstrated certain degradation of beta-TCP and extensive new bone filling in rich extracellular matrix after 6 weeks. The cells were stained positively for type I collagen. After 12 weeks, the bioceramics had almost completely degraded and abundant bone formation could be seen in the whole defects. In group B, marginal bone ingrowth was observed after 6 weeks and the number of osteoblasts increased significantly after 12 weeks. However, no new bone formation could be detected in the middle of the material. In group C, only a small quantity of new bone formation was found along the margin of defects.
Transplantation of MSCs with beta-TCP can serve as an example of a cell-based treatment for bone regeneration in skeletal defects.
确定体外培养扩增的骨髓间充质干细胞（MSCs）与β-磷酸三钙（β-TCP）联合应用能否修复新西兰兔的严重颅骨缺损。
A组（n = 20），从同系兔分离MSCs并在体外扩增，然后植入预成型的多孔β-TCP上。将MSCs-β-TCP复合物植入兔严重颅骨缺损处。B组（n = 10），仅用β-TCP修复缺损。C组（n = 4），缺损不做处理。术后6周和12周取材进行组织学、组织化学和免疫组织化学分析。
A组，植入物表面可见类骨组织形成。显微镜分析显示，6周后β-TCP有一定程度降解，富含细胞外基质中有大量新骨填充。细胞I型胶原染色呈阳性。12周后，生物陶瓷几乎完全降解，整个缺损处可见大量骨形成。B组，6周后观察到边缘骨长入，12周后成骨细胞数量显著增加。然而，材料中部未检测到新骨形成。C组，仅在缺损边缘发现少量新骨形成。
MSCs与β-TCP联合移植可作为骨骼缺损骨再生细胞治疗的一个范例。
