Xu Songfeng, Lin Kaili, Wang Zhen, Chang Jiang, Wang Lin, Lu Jianxi, Ning Congqin
Institute of Orthopedic Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
Biomaterials. 2008 Jun;29(17):2588-96. doi: 10.1016/j.biomaterials.2008.03.013. Epub 2008 Apr 1.
In this study, the in vivo bone-regenerative capacity and resorption of the porous beta-calcium silicate (beta-CaSiO(3), beta-CS) bioactive ceramics were investigated in a rabbit calvarial defect model, and the results were compared with porous beta-tricalcium phosphate (beta-Ca(3)(PO(4))(2), beta-TCP) bioceramics. The porous beta-CS and beta-TCP ceramics were implanted in rabbit calvarial defects and the specimens were harvested after 4, 8 and 16 weeks, and evaluated by Micro-CT and histomorphometric analysis. The Micro-CT and histomorphometric analysis showed that the resorption of beta-CS was much higher than that of beta-TCP. The TRAP-positive multinucleated cells were observed on the surface of beta-CS, suggesting a cell-mediated process involved in the degradation of beta-CS in vivo. The amount of newly formed bone was also measured and more bone formation was observed with beta-CS as compared with beta-TCP (p<0.05). Histological observation demonstrated that newly formed bone tissue grew into the porous beta-CS, and a bone-like apatite layer was identified between the bone tissue and beta-CS materials. The present studies showed that the porous beta-CS ceramics could stimulate bone regeneration and may be used as bioactive and biodegradable materials for hard tissue repair and tissue engineering applications.
在本研究中，在兔颅骨缺损模型中研究了多孔β-硅酸钙（β-CaSiO₃，β-CS）生物活性陶瓷的体内骨再生能力和吸收情况，并将结果与多孔β-磷酸三钙（β-Ca₃(PO₄)₂，β-TCP）生物陶瓷进行比较。将多孔β-CS和β-TCP陶瓷植入兔颅骨缺损处，在4、8和16周后采集标本，并通过显微CT和组织形态计量学分析进行评估。显微CT和组织形态计量学分析表明，β-CS的吸收远高于β-TCP。在β-CS表面观察到TRAP阳性多核细胞，表明体内β-CS的降解涉及细胞介导的过程。还测量了新形成骨的量，与β-TCP相比，β-CS观察到更多的骨形成（p<0.05）。组织学观察表明，新形成的骨组织长入多孔β-CS中，并且在骨组织和β-CS材料之间鉴定出类骨磷灰石层。本研究表明，多孔β-CS陶瓷可以刺激骨再生，并且可用作硬组织修复和组织工程应用的生物活性和可生物降解材料。
