Yang Ying-yong, Yu Shu-xian, Jia Ji-zhang
China Leather and Footwear Industry Research Institute, Beijing, P. R. China, 100016.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2003 Jul;17(4):339-42.
To build the trestle of tissue engineering for skin with the collagen.
The collagen was obtained from the baby cattle hide pretreated by Na2S and elastinase and Protease M, then the collagen was dissolved in 0.5 mol/L acetic acid solution. The collagen was treated with Protease N to minimize its immunogenicity. The resulting collagen could be used to build the trestle of tissue engineering for skin because of good biocompatibility. The collagen molecular weight and structure were analyzed by SDS-PAGE. The bioactivity of trestle was tested in the experiment of the mice wound healing and the cell implantation.
The SDS-PAGE result of the collagen treated by Protease M showed the typical spectrum of type I collagen. The built trestle was a collagen sponge matrix in which micropore size was 50-200 microns. It could accelerate wound healing and the implanted fibroblasts could proliferate well.
The collagen treated by Protease N can get good biocompatibilily and is suitable for building the trestles of tissue engineering for skin with good bioactivity.
用胶原蛋白构建皮肤组织工程支架。
从经硫化钠、弹性蛋白酶和蛋白酶M预处理的小牛皮中获取胶原蛋白，然后将其溶解于0.5mol/L乙酸溶液中。用蛋白酶N处理胶原蛋白以使其免疫原性降至最低。所得胶原蛋白因其良好的生物相容性可用于构建皮肤组织工程支架。通过SDS-PAGE分析胶原蛋白的分子量和结构。在小鼠伤口愈合实验和细胞植入实验中测试支架的生物活性。
经蛋白酶M处理的胶原蛋白的SDS-PAGE结果显示出典型的I型胶原图谱。构建的支架为胶原海绵基质，其微孔尺寸为50 - 200微米。它可加速伤口愈合，植入的成纤维细胞能良好增殖。
经蛋白酶N处理的胶原蛋白具有良好的生物相容性，适合构建具有良好生物活性的皮肤组织工程支架。
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