Doillon C J, Silver F H, Olson R M, Kamath C Y, Berg R A
Department of Pathology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway 08854.
Scanning Microsc. 1988 Jun;2(2):985-92.
Fibroblast and epidermal cell-type I collagen sponge interactions were studied in cell culture as well as in humans. In cell culture, fibroblasts were observed to migrate and proliferate throughout a type I collagen sponge containing either hyaluronic acid (HA) or fibronectin (FN). Fibroblasts accumulated in the center of the pores in sponges containing HA and appeared to surround themselves with newly synthesized extracellular matrix. In sponges containing FN, fibroblasts attached to and elongated along the collagen fibers of the sponge. In the absence of FN or HA protein synthesis of fibroblasts appeared to be inhibited by the presence of the type I collagen sponge. Epidermal cells grown on plastic or on type I collagen, formed sheets. Epidermal cells grown on a collagen sponge morphologically appeared different than cells grown on plastic. The type I collagen matrix studied in cell culture was applied to dermal wounds of patients with pressure ulcers in order to evaluate its effect on dermal wound healing. The areas of ulcers treated for 6 weeks with a type I collagen sponge decreased by about 40% compared with no change in the areas of untreated controls. Preliminary results suggest that a type I collagen sponge is a biocompatible substrate with fibroblasts and epidermal cells and may be effective in enhancing healing of chronic skin ulcers.
在细胞培养以及人体中研究了成纤维细胞与表皮细胞和I型胶原海绵的相互作用。在细胞培养中，观察到成纤维细胞在含有透明质酸（HA）或纤连蛋白（FN）的I型胶原海绵中迁移和增殖。在含有HA的海绵中，成纤维细胞聚集在孔隙中心，并似乎被新合成的细胞外基质包围。在含有FN的海绵中，成纤维细胞附着在海绵的胶原纤维上并沿其伸长。在没有FN或HA的情况下，I型胶原海绵的存在似乎抑制了成纤维细胞的蛋白质合成。在塑料或I型胶原上生长的表皮细胞形成片状。在胶原海绵上生长的表皮细胞在形态上与在塑料上生长的细胞不同。将在细胞培养中研究的I型胶原基质应用于压疮患者的皮肤伤口，以评估其对皮肤伤口愈合的影响。与未治疗的对照组伤口面积无变化相比，用I型胶原海绵治疗6周的溃疡面积减少了约40%。初步结果表明，I型胶原海绵是一种与成纤维细胞和表皮细胞具有生物相容性的基质，可能对促进慢性皮肤溃疡的愈合有效。
