Chen Ying-hua, Dong Wei-ren, Xiao Ying-qing, Zhao Bing-lei, Hu Guo-dong, An Lian-bing
Department of Histology and Embryology, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Feb;26(2):131-8.
To develop a three-dimensional porous film of human hair keratin (HHK)-collagen sponge complex for use as a dermal substitute.
The three components F, B, and Z derived from healthy human hair were weaved into a meshwork and integrated with purified soluble type I collagen extracted from bovine tendons to prepare a highly porous film with vacuum freeze-drying followed by secondary cross-linking with glutaraldehyde. The film was grafted beneath the dorsal skin in 21 SD rats (experimental group), with simple collagen sponge serving as the negative control. The rats receiving surgical operation but without graft served as the blank control. The graft and its surrounding tissue were harvested on days 3, 7 and at weeks 2, 4, 6, 8, 12 after implantation for evaluation of tissue compatibility, vascularization and degradation.
The prepared collagen sponge film was semitransparent and porous. Three to 7 days after grafting, inflammatory reaction was relieved gradually, and several fibroblasts and blood vessels were found adherent to the grafts in the experimental groups. At week 4, the wounds healed in the experimental groups, and the fibroblasts were actively secreting collagen and the film degraded obviously with the appearance of elastic fibers. At weeks 6 and 8, new collagen fibers thickened and assumed regular arrangement, and the collagen sponge films disappeared completely. In the control groups, the changes were less obvious and total HHK degradation occurred till week 12.
The degradable and absorbable HHK-collagen sponge film has relatively satisfactory tissue compatibility and can accelerate wound healing by stimulating cell proliferation and vascularization, showing the potential as an optimal dermal substitute.
制备一种人发角蛋白（HHK）-胶原海绵复合物三维多孔膜用作真皮替代物。
将来源于健康人发的三种成分F、B和Z编织成网状结构，并与从牛肌腱中提取的纯化可溶性I型胶原整合，经真空冷冻干燥，再用戊二醛进行二次交联，制备出高度多孔膜。将该膜移植到21只SD大鼠的背部皮肤下（实验组），单纯胶原海绵作为阴性对照。接受手术但未移植的大鼠作为空白对照。在植入后第3天、第7天以及第2周、第4周、第6周、第8周、第12周采集移植物及其周围组织，评估组织相容性、血管化和降解情况。
制备的胶原海绵膜呈半透明且多孔状。移植后3至7天，炎症反应逐渐减轻，实验组移植物上可见一些成纤维细胞和血管附着。第4周时，实验组伤口愈合，成纤维细胞积极分泌胶原，膜明显降解并出现弹性纤维。第6周和第8周时，新的胶原纤维增粗并呈规则排列，胶原海绵膜完全消失。对照组变化较不明显，直至第12周HHK才完全降解。
可降解、可吸收的HHK-胶原海绵膜具有相对满意的组织相容性，可通过刺激细胞增殖和血管化促进伤口愈合，显示出作为理想真皮替代物的潜力。
