Wang Hongwei, Shang Lanqin, Hao Weidong
Department of Toxicology, Peking University School of Public Health, Beijing 100083, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Feb 18;35(1):69-73.
To analyse the induction of apoptosis and reactive oxygen species (ROS) in human skin fibroblast by ultraviolet A (UVA).
Human skin fibroblasts were irradiated with UVA, apoptosis was determined with flowcytometry and fluorescent microscopy, the intracellular ROS production was measured with a spectrofluorometer, and the free radicals were determined with electron spin resonance.
A significant increase of apoptosis was observed in human skin fibroblast after exposure to UVA with a dosage at 110-120 kJ.m-2, mannitol could counteract apoptosis; a higher level of intracellular ROS and the hydroxyl free radical was detected in the irradiated samples.
A lower dose of irradiation of UVA(0.6 mW.cm-2) can induce apoptosis in human skin fibroblast, and ROS might play a role in the induction of apoptosis.
分析紫外线A（UVA）对人皮肤成纤维细胞凋亡及活性氧（ROS）的诱导作用。
用UVA照射人皮肤成纤维细胞，采用流式细胞术和荧光显微镜检测细胞凋亡，用荧光分光光度计测定细胞内ROS生成量，用电子自旋共振测定自由基。
人皮肤成纤维细胞在接受剂量为110 - 120 kJ·m-2的UVA照射后，凋亡显著增加，甘露醇可对抗凋亡；照射后的样本中检测到细胞内ROS和羟自由基水平较高。
较低剂量的UVA（0.6 mW·cm-2）照射可诱导人皮肤成纤维细胞凋亡，ROS可能在凋亡诱导中起作用。
