Leite-Silva Cleide, Santos Neide, Fagundes Valéria, Yonenaga-Yassuda Yatiyo, de Souza Maria José
Departamento de Genética/CCB, Universidade Federal de Pernambuco, Cidade Universitária, Recife, PE, Brazil.
Hereditas. 2003;138(2):94-100. doi: 10.1034/j.1601-5223.2003.01693.x.
The karyotypes of the bat species Molossus ater, M. molossus (2n = 48; NF = 64) and Molossops planirostris (2n = 34; NF = 60) were analyzed by G-, C-banding, silver nitrate staining (AgNO3), base-specific fluorochromes and fluorescent in situ hybridization (FISH). The two species of Molossus presented the constitutive heterochromatin (CH) in the pericentromeric regions of all autosomes and in the X chromosome, while the Y chromosome was completely heterochromatic. Molossops planirostris showed conspicuous CH blocks in the pericentromeric regions of the pairs 4, 5, 8, 15, 16 and in the short arm of the X chromosome, while the Y did not present any CH block. Pretreated slides for C-banding stained with DAPI (CB-DAPI) revealed a similar pattern of C-banding (CBG) for these species. Sequential staining (AgNO3/CMA3/DAPI) in M. planirostris showed that the nucleolus organizer regions (NORs) are weakly CMA3 positive and DAPI negative. In the three species, triple staining with CMA3/DA/DAPI revealed R-banding with CMA3 and uniform staining with DAPI. The ribosomal cistrons detected by FISH were present only in the pair 5 in both species of Molossus, and in two pairs of medium sized chromosomes (pairs 9 and 10) of Molossops planirostris. The results obtained by FISH, compared with those by AgNO3 staining, indicated that all NORs in these species are transcriptionally active.
采用G显带、C显带、硝酸银染色（AgNO₃）、碱基特异性荧光染料和荧光原位杂交（FISH）技术，对黑犬吻蝠、犬吻蝠（2n = 48；NF = 64）和平脸犬吻蝠（2n = 34；NF = 60）的核型进行了分析。犬吻蝠属的两个物种在所有常染色体的着丝粒周围区域和X染色体上均呈现组成型异染色质（CH），而Y染色体完全是异染色质。平脸犬吻蝠在4、5、8、15和16号染色体对的着丝粒周围区域以及X染色体短臂上显示出明显的CH块，而Y染色体上没有任何CH块。用DAPI染色的C显带预处理玻片（CB-DAPI）显示这些物种的C显带（CBG）模式相似。平脸犬吻蝠的顺序染色（AgNO₃/CMA₃/DAPI）表明，核仁组织区（NORs）对CMA₃呈弱阳性，对DAPI呈阴性。在这三个物种中，CMA₃/DA/DAPI三重染色显示CMA₃呈R显带，DAPI呈均匀染色。通过FISH检测到的核糖体顺反子仅存在于犬吻蝠属的两个物种的5号染色体对中，以及平脸犬吻蝠的两对中等大小染色体（9号和10号染色体对）中。与AgNO₃染色结果相比，FISH获得的结果表明这些物种中的所有NORs都具有转录活性。
