Santos N, Fagundes V, Yonenaga-Yassuda Y, De Souza M J
Departamento de Genética/CCB, Universidade Federal de Pernambuco, Cidade Universitária, Recife, Pernambuco, 50732-970, Brazil.
Hereditas. 2001;134(3):189-94. doi: 10.1111/j.1601-5223.2001.00189.x.
This paper provides new data on chromosomes of Brazilian vampire bats Desmodus rotundus and Diphylla ecaudata. These species were analyzed by GTG, CBG- and CB-DAPI banding, AgNO3/CMA3 sequential staining, base-specific fluorochrome dyes and in situ hybridization with 18S rDNA probe. C-banding (CBG) revealed constitutive heterochromatin in the pericentromeric regions in all autosomes and the X and Y chromosomes appeared entirely heterochromatic in both species. CB-DAPI revealed a coincident banding pattern to that obtained by CBG. Triple staining CMA3/DA/DAPI revealed an R-banding and a weak G-banding pattern in the karyotypes. Sequential AgNO3/CMA3 staining showed a NOR located interstitially on the long arm of pair 8 in D. rotundus and on the short arm of pair 13 in D. ecaudata. FISH with a rDNA probe confirmed the location and number of NORs; a difference neither in intensity nor in size of hybridization signal was detected between homologues for both species.
本文提供了关于巴西吸血蝙蝠圆叶吸血蝠（Desmodus rotundus）和无尾叶鼻蝠（Diphylla ecaudata）染色体的新数据。通过GTG、CBG和CB-DAPI显带、AgNO3/CMA3顺序染色、碱基特异性荧光染料以及用18S rDNA探针进行原位杂交对这些物种进行了分析。C显带（CBG）显示，所有常染色体的着丝粒周围区域均存在组成型异染色质，在这两个物种中，X和Y染色体均呈现完全异染色质状态。CB-DAPI显示出与CBG获得的带型模式一致。三重染色CMA3/DA/DAPI在核型中显示出一种R显带和一种较弱的G显带模式。AgNO3/CMA3顺序染色显示，圆叶吸血蝠的第8对染色体长臂中间位置以及无尾叶鼻蝠的第13对染色体短臂中间位置存在核仁组织区（NOR）。用rDNA探针进行的荧光原位杂交（FISH）证实了NOR的位置和数量；在这两个物种的同源染色体之间，未检测到杂交信号在强度或大小上的差异。
