人类胚胎干细胞的分子特征及其与小鼠的比较。
Molecular signature of human embryonic stem cells and its comparison with the mouse.
作者信息
Sato Noboru, Sanjuan Ignacio Munoz, Heke Michael, Uchida Makiko, Naef Felix, Brivanlou Ali H
机构信息
Laboratory of Vertebrate Molecular Embryology, The Rockefeller University, 1230, York Avenue, New York, NY 10021, USA.
出版信息
Dev Biol. 2003 Aug 15;260(2):404-13. doi: 10.1016/s0012-1606(03)00256-2.
The molecular mechanism underlying pluripotency is largely unknown. Here, we provide the first global transcriptional profile of the state of "stemness" in human embryonic stem cells (HESCs). We have identified a set of 918 genes enriched in undifferentiated HESCs compared with their differentiated counterparts. These include ligand/receptor pairs and secreted inhibitors of the FGF, TGFbeta/BMP, and Wnt pathways, highlighting a prevalent role for these pathways in HESCs. Importantly, a significant number of HESCs-enriched genes, including several signaling components, are found to be intersected with published mouse embryonic stem cell data, indicating that a "core molecular program" is shared between the two pluripotent stem cells.
多能性背后的分子机制在很大程度上尚不清楚。在此，我们提供了人类胚胎干细胞（hESC）“干性”状态的首个全基因组转录图谱。我们已经鉴定出一组918个基因，与分化后的hESC相比，这些基因在未分化的hESC中富集。这些基因包括配体/受体对以及FGF、TGFβ/BMP和Wnt信号通路的分泌抑制剂，突出了这些信号通路在hESC中的普遍作用。重要的是，发现大量hESC富集基因，包括几个信号成分，与已发表的小鼠胚胎干细胞数据相交，表明这两种多能干细胞之间共享一个“核心分子程序”。
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