Bae Jong-Lye, Lee Jong-Gul, Kang Tae-Jin, Jang Hyon-Seok, Jang Yong-Suk, Yang Moon-Sik
Division of Biological Sciences, Institute for Molecular Biology and Genetics, Chonbuk National University, Chonju Dukjindiong 664-14, Chonju 561-756, South Korea.
Vaccine. 2003 Sep 8;21(25-26):4052-8. doi: 10.1016/s0264-410x(03)00360-8.
A report from that the presence of lactogenic immunity in pigs protected suckling piglets from porcine epidemic diarrhea virus (PEDV) infection suggested that inducing mucosal immune responses in lactating pigs is an effective way of protecting swine from PEDV infection. In this study, we developed transgenic tobacco plants that express the antigen protein corresponding to the neutralizing epitope of PEDV spike protein, and tested whether feeding the plants to pigs induced an effective immune response against PEDV infection. First, we confirmed the immunogenicity of the plant-derived antigen by using a plaque reduction neutralization assay with serum obtained after injecting mice with protein extracted from the transgenic plants. Feeding the transgenic plants to mice induced both systemic and mucosal immune responses against the antigen. The induced antibodies inhibited virus infection in the plaque reduction neutralization assay. These results suggest that feeding animals transgenic plants carrying antigen genes is an effective strategy to induce protective immune responses against PEDV infection.
一份报告指出，猪体内的泌乳免疫可保护哺乳仔猪免受猪流行性腹泻病毒（PEDV）感染，这表明在泌乳母猪中诱导黏膜免疫反应是保护猪免受PEDV感染的有效方法。在本研究中，我们培育了表达与PEDV刺突蛋白中和表位相对应的抗原蛋白的转基因烟草植株，并测试了将这些植株喂给猪是否能诱导针对PEDV感染的有效免疫反应。首先，我们通过空斑减少中和试验，利用注射从转基因植株中提取的蛋白质后获得的血清，确认了植物源抗原的免疫原性。将转基因植株喂给小鼠可诱导针对该抗原的全身和黏膜免疫反应。在空斑减少中和试验中，诱导产生的抗体可抑制病毒感染。这些结果表明，给动物喂食携带抗原基因的转基因植株是诱导针对PEDV感染的保护性免疫反应的有效策略。
