Ryu Jin-Woo, Kim Hyung-Jee, Lee Young-Sun, Myong Na-Hye, Hwang Cheol-Hoh, Lee Gae-Sung, Yom Heng-Cherl
Research Group of Proteomics, Breast Clinic of Ewha Woman Hospital, Korea.
J Korean Med Sci. 2003 Aug;18(4):505-9. doi: 10.3346/jkms.2003.18.4.505.
Gastric cancer is a very serious disease and is naturally resistant to many anticancer drugs. To reduce the mortality and improve the effectiveness of therapy, many studies have tried to find key biomarkers. Proteomic technologies are providing the tools needed to discover and identify disease-associating biomarkers. The proteomic study of gastric cancer establishes any specific events that lead to cancer, and it provides a direct way to define the true function of genes. Using two dimensional (2-D) electrophoresis of the stomach cancer tissue, we have gained about 1,500 spots in each gel, and 140 protein spots also were identified. Among the identified proteins, there were seven over-expressed proteins in stomach cancer tissue: NSP3, transgelin, prohibitin, heat shock protein (hsp) 27 and variant, protein disulfide isomerase A3, unnamed protein product and glucose regulated protein. There were also seven under-expressed proteins in stomach cancer: Apolipoprotein A-1, p20, nucleoside diphosphate isomerase A, alpha 1 antitrypsin, desmin, serum albumin and serotransferrin.
胃癌是一种非常严重的疾病，对许多抗癌药物天然耐药。为了降低死亡率并提高治疗效果，许多研究试图寻找关键生物标志物。蛋白质组学技术为发现和鉴定与疾病相关的生物标志物提供了所需的工具。胃癌的蛋白质组学研究确定了导致癌症的任何特定事件，并提供了一种直接的方法来定义基因的真正功能。通过对胃癌组织进行二维（2-D）电泳，我们在每块凝胶上获得了约1500个斑点，还鉴定出了140个蛋白质斑点。在鉴定出的蛋白质中，有七种在胃癌组织中过度表达：NSP3、转胶蛋白、抑制素、热休克蛋白（hsp）27及其变体、蛋白质二硫键异构酶A3、未命名蛋白质产物和葡萄糖调节蛋白。胃癌中还有七种表达不足的蛋白质：载脂蛋白A-1、p20、核苷二磷酸异构酶A-α1抗胰蛋白酶、结蛋白、血清白蛋白和转铁蛋白。
