Moodie Elizabeth G, Le Jambre Leo F, Katz Margaret E
Zoology, School of Biological, Biomedical and Molecular Sciences, University of New England, NSW 2351 Armidale, Australia.
Parasitol Res. 2003 Sep;91(2):151-65. doi: 10.1007/s00436-003-0941-3. Epub 2003 Aug 16.
Thelohania parastaci sp. nov. infects the Australian freshwater crayfish, Cherax destructor. Data on morphology, developmental patterns and sequences from the small subunit (SSU) and internal transcribed spacer (ITS) regions of the ribosomal DNA (rDNA) of T. parastaci sp. nov. are described. The ultrastructural features of different life cycle stages are very similar to those of the European crayfish parasite Thelohania contejeani. T. parastaci sp. nov. exhibits simultaneous dimorphic sporogony in muscle tissue. Meronts, sporonts and spores are found in muscle tissue, within haemocytes in the hepatopancreas, and in the intestinal wall of infected crayfish. T. parastaci sp. nov. shows 92% sequence identity with T. contejeani and only 67% sequence identity with the fire ant pathogen T. solenopsae, when SSU rDNA sequences are compared. Analysis of SSU rDNA and ITS sequences of T. parastaci sp. nov. from crayfish from Victoria, Western Australia, and New South Wales indicate that the parasite has a wide geographical distribution in Australia.
副斯塔奇嗜碘孢菌（Thelohania parastaci sp. nov.）感染澳大利亚淡水小龙虾——破坏螯虾（Cherax destructor）。本文描述了副斯塔奇嗜碘孢菌的形态学、发育模式以及核糖体DNA（rDNA）的小亚基（SSU）和内部转录间隔区（ITS）的序列数据。不同生命周期阶段的超微结构特征与欧洲小龙虾寄生虫康氏嗜碘孢菌（Thelohania contejeani）非常相似。副斯塔奇嗜碘孢菌在肌肉组织中表现出同步双态孢子形成。裂殖体、孢子体和孢子存在于肌肉组织、肝胰腺的血细胞内以及受感染小龙虾的肠壁中。当比较SSU rDNA序列时，副斯塔奇嗜碘孢菌与康氏嗜碘孢菌的序列同一性为92%，与火蚁病原体索氏嗜碘孢菌（T. solenopsae）的序列同一性仅为67%。对来自维多利亚州、西澳大利亚州和新南威尔士州小龙虾的副斯塔奇嗜碘孢菌的SSU rDNA和ITS序列分析表明，该寄生虫在澳大利亚具有广泛的地理分布。
