Moodie Elizabeth G, Le Jambre Leo F, Katz Margaret E
Zoology, School of Biological, Biomedical and Molecular Sciences, University of New England, NSW 2351, Armidale, Australia.
Parasitol Res. 2003 Oct;91(3):215-28. doi: 10.1007/s00436-003-0948-9. Epub 2003 Aug 16.
Thelohania montirivulorum sp. nov., a new species of microsporidian parasite, was found in a highland population of the Australian yabby, Cherax destructor. Data are presented on fine ultrastructure, developmental morphology and DNA sequence of the small subunit ribosomal DNA (SSU rDNA) and internal transcribed spacer region. The phylogenetic relationships of T. montrivulorum sp. nov. and other crayfish parasites in the genus Thelohania, based on the SSU rDNA sequence, are investigated. Fine ultrastructure, patterns of sporogony and SSU rDNA sequence similarities indicate T. montirivulorum sp. nov. is congeneric with T. parastaci, a parasite of lowland populations of C. destructor, and with T. contejeani, a parasite of European freshwater crayfish. SSU rDNA data suggests Thelohania species found in crustacean hosts are more closely related to the Vairimorpha/ Nosema clade of species from insect and crustacean hosts than to the fire ant parasites, T. solenopsae and Thelohania sp.
新种蒙蒂里武洛氏微孢子虫（Thelohania montirivulorum sp. nov.）是在澳大利亚螯虾（Cherax destructor）的高地种群中发现的一种新型微孢子虫寄生虫。本文呈现了该寄生虫的精细超微结构、发育形态以及小亚基核糖体DNA（SSU rDNA）和内部转录间隔区的DNA序列数据。基于SSU rDNA序列，研究了蒙蒂里武洛氏微孢子虫与Thelohania属其他小龙虾寄生虫的系统发育关系。精细超微结构、孢子生殖模式和SSU rDNA序列相似性表明，蒙蒂里武洛氏微孢子虫与低地种群澳大利亚螯虾的寄生虫副斯塔氏微孢子虫（T. parastaci）以及欧洲淡水小龙虾的寄生虫孔泰贾尼氏微孢子虫（T. contejeani）同属。SSU rDNA数据表明，在甲壳类宿主中发现的Thelohania物种与来自昆虫和甲壳类宿主的Vairimorpha/Nosema类群的物种关系更为密切，而与火蚁寄生虫索氏微孢子虫（T. solenopsae）和Thelohania sp.关系较远。
