[利用微卫星标记定位T型细胞质雄性不育小麦育性恢复基因的位置]

[Location of the fertility restorer gene for T-type CMS wheat by microsatellite marker].

作者信息

Zhang Cui, Wang Hong-Ying, Shen Yin-Zhu, Zhao Bao-Cun, Zhu Zheng-Ge, Huang Zhan-Jing

机构信息

College of Life Sciences, Heibe Normal University, Shijiazhuang 050016, China.

出版信息

Yi Chuan Xue Bao. 2003 May;30(5):459-64.

PMID:12924161

Abstract

Through the genetic analysis of a F2 population, derived from CMS line 75-3369A (T-type CMS wheat) and the restorer line 7269-10, the result indicated that the restorer line was conditioned by two dominant genes. A F2 population was used to map the fertility restorer (Rf) gene by microsatellite and BSA (bulked segregant analysis). Restorer and sterile DNA pools were established using the extreme fertile and sterile plants of F2 population, respectively. Among the 230 pairs of microsatellite primers, two markers were found polymorphic between the two pools. Linkage analysis showed that microsatellite marker Xgwm136 and Xgwm550 were linked with the two fertility restorer genes, respectively. One of the Rf gene was located on 1AS and the genetic distance between the SSR marker Xgwm136 and this Rf gene was 6.7 cM, the other Rf gene was located on 1BS and with a genetic distance of 5.1 cM to marker Xgwm550.

摘要

通过对由细胞质雄性不育系75 - 3369A（T型细胞质雄性不育小麦）和恢复系7269 - 10构建的F2群体进行遗传分析，结果表明该恢复系受两个显性基因控制。利用一个F2群体，通过微卫星标记和混合分组分析法（BSA）对育性恢复（Rf）基因进行定位。分别使用F2群体中极端可育和不育植株建立了恢复系和不育系DNA池。在230对微卫星引物中，发现有两个标记在两个DNA池之间表现出多态性。连锁分析表明，微卫星标记Xgwm136和Xgwm550分别与两个育性恢复基因连锁。其中一个Rf基因位于1AS上，SSR标记Xgwm136与该Rf基因之间的遗传距离为6.7 cM，另一个Rf基因位于1BS上，与标记Xgwm550的遗传距离为5.1 cM。