Weng Dong-Xu, Xu Shi-Chang, Lin Rui-Ming, Wan An-Min, Li Jing-Peng, Wu Li-Ren
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, State Key Lab of Biology for Plant Diseases and Insect Pests , Beijing 100094, China.
Yi Chuan Xue Bao. 2005 Sep;32(9):937-41.
SSR analysis was performed using a wheat near-isogenic line (NIL) Taichuang29 * 6/ Lovrin13, which carried the resistance gene Yr9 against wheat stripe rust and its recurrent parent Taichung29 as materials. After screening with 32 SSR primers on 1B chromosome, reproducible polymorphic DNA fragment amplified by Xgwm582 was identified. Genetic linkage was tested in 177 segregating F2 plants. The results indicated that microsatellite marker Xgwm582 was linked with gene Yr9 resistant to wheat stripe rust. A genetic distance of 3. 7 cM was calculated.
以携带抗小麦条锈病基因Yr9的小麦近等基因系（NIL）太中29*6/Lovrin13及其轮回亲本太中29为材料进行简单序列重复（SSR）分析。用32对位于1B染色体上的SSR引物进行筛选后，鉴定出由Xgwm582扩增出的可重复多态性DNA片段。在177株分离的F2植株中进行遗传连锁检测。结果表明，微卫星标记Xgwm582与抗小麦条锈病基因Yr9连锁。计算出的遗传距离为3.7厘摩。
