Burgemeister Renate, Gangnus Rainer, Haar Beate, Schütze Karin, Sauer Ulrich
P.A.L.M. Microlaser Technologies AG, Bernried, Germany.
Pathol Res Pract. 2003;199(6):431-6. doi: 10.1078/0344-0338-00442.
Isolation of intact RNA in high quality is the first and often the most critical step in performing many fundamental molecular biology experiments, and is essential for many techniques used in gene expression analysis. As many factors influence nucleic acid preservation, RNA isolation should include some important steps before and after the actual RNA extraction. We tested the influence of fixation and staining protocols regarding RNA integrity and concentration. A factor that is often underestimated is the absolute necessity for homogenous starting materials. Application of the LMPC technology allows for a rapidand highly precise procurement of purified cell populations suitable for a variety of downstream analyses.
高质量完整RNA的分离是进行许多基础分子生物学实验的首要且往往是最关键的步骤，对于基因表达分析中使用的许多技术而言至关重要。由于许多因素会影响核酸的保存，RNA分离在实际RNA提取前后应包括一些重要步骤。我们测试了固定和染色方案对RNA完整性和浓度的影响。一个经常被低估的因素是起始材料均匀性的绝对必要性。LMPC技术的应用能够快速且高度精确地获取适合各种下游分析的纯化细胞群体。
