Nafe Reinhold, Gangnus Rainer, Glienke Wolfgang, Burgemeister Renate, Haar Beate, Pries Antje, Schlote Wolfgang
Department Neuroradiology, Clinics of Goethe-University, Frankfurt am Main, Germany.
Pathol Res Pract. 2003;199(6):411-4. doi: 10.1078/0344-0338-00438.
The technique of laser microdissection together with laser pressure catapulting (LMPC) is demonstrated in paraffin sections obtained from surgical specimens of brain tumors mounted on glass slides. A sufficient and precise application of microdissection techniques in tissue on glass slides is worthwhile, since it offers the possibility of a retrospective analysis of archived paraffin sections in histopathology. We could demonstrate a precise dissection of areas in tissues of different thicknesses (4 microm and 20 microm). Areas of tissue mounted directly on glass need to be dissected in a scanning mode in order to remove the total region in form of small tissue fragments row by row. This mode provided a precise microdissection of tissue areas of different sizes and shapes. A successful molecular biological analysis of the microdissected regions could be demonstrated. As an example for such an analysis, differential-PCR for detecting an amplification of the gene for the epidermal growth factor receptor (EGFR) was performed.
在安装于载玻片上的脑肿瘤手术标本的石蜡切片中演示了激光显微切割技术以及激光压力弹射技术（LMPC）。在载玻片上的组织中充分且精确地应用显微切割技术是值得的，因为它为组织病理学中存档石蜡切片的回顾性分析提供了可能性。我们能够证明对不同厚度（4微米和20微米）的组织区域进行精确切割。直接安装在载玻片上的组织区域需要以扫描模式进行切割，以便逐行以小组织碎片的形式去除整个区域。这种模式为不同大小和形状的组织区域提供了精确的显微切割。可以证明对显微切割区域进行了成功的分子生物学分析。作为这种分析的一个例子，进行了用于检测表皮生长因子受体（EGFR）基因扩增的差异PCR。
