Yang J, Yang F Y
National Laboratory of Biomacromolecules, Institute of Biophysics, Academia Sinica, Beijing, China.
Biofactors. 1992 Dec;4(1):29-32.
The transport activity of Band 3 of spectrin-stripped inside-out erythrocyte membrane vesicles (IOVs) or resealed ghosts was enhanced in the presence of trace amounts of Na2SeO3 (0.2-0.5 p.p.m.); however, at higher concentrations of Na2SeO3 (> 4.0 p.p.m.), an inverse result was obtained. Reassociation of spectrin with IOVs has no effect either on the transport activity of Band 3 or on the enhancement of its activity by Na2SeO3. Sulfhydryl reagents (p-chloromercuribenzoic acid and N-ethylmaleimide) could also inhibit Band 3 activity and eliminate the selenium effect. It is suggested that SH groups are involved in anion transport of Band 3 and that the selenium effect is based on the interaction of SH groups of Band 3 with Na2SeO3.
在存在痕量亚硒酸钠（0.2 - 0.5 ppm）的情况下，血影蛋白去除的内翻红细胞膜囊泡（IOV）或重封血影的带3转运活性增强；然而，在较高浓度的亚硒酸钠（> 4.0 ppm）下，得到了相反的结果。血影蛋白与IOV的重新结合对带3的转运活性或亚硒酸钠对其活性的增强均无影响。巯基试剂（对氯汞苯甲酸和N - 乙基马来酰亚胺）也可抑制带3活性并消除硒效应。提示巯基参与带3的阴离子转运，且硒效应基于带3的巯基与亚硒酸钠的相互作用。
