Grønlund B, Grønlund L, Madsbad S, Fogh-Andersen N
Department of Internal Medicine & Endocrinology, Herlev Hospital, University of Copenhagen, Denmark.
Biosens Bioelectron. 1992;7(10):683-7. doi: 10.1016/0956-5663(92)85049-g.
A new prototype direct reading glucose electrode working with glucose oxidase and hydrogen peroxide was preliminarily tested clinically during insulin-induced hypoglycemia in eight healthy subjects, and during hyperglycemia in five dysregulated diabetic patients. The results for 282 whole blood samples were compared to those of our routine method, which measures the glucose concentration in whole blood. The correlation was: y = 1.05.x - 0.05 mmol/L, r = 0.99. The glucose electrode measured a glucose concentration of 10.5 mmol/L +- 0.49 mmol/L (between-day imprecision) in a control serum (glucose 10.0 mmol/L). The glucose electrode supposedly responds to the activity of glucose that equals the molality (mmol glucose per kg water). The ratio of results with the glucose electrode and our routine method was lower than the expected ratio between water concentration in calibrator and whole blood, which is 1.18. A steep gradient from blood sample to glucose electrode, depending on the diffusion coefficient and hematocrit might explain the discrepancy.
一种使用葡萄糖氧化酶和过氧化氢的新型直接读数葡萄糖电极，在8名健康受试者胰岛素诱导的低血糖期间以及5名血糖失调的糖尿病患者的高血糖期间进行了初步临床测试。将282份全血样本的结果与我们测量全血葡萄糖浓度的常规方法的结果进行了比较。相关性为：y = 1.05x - 0.05 mmol/L，r = 0.99。在对照血清（葡萄糖10.0 mmol/L）中，葡萄糖电极测得的葡萄糖浓度为10.5 mmol/L ± 0.49 mmol/L（日间不精密度）。该葡萄糖电极据称对等于质量摩尔浓度（每千克水所含葡萄糖的毫摩尔数）的葡萄糖活性作出反应。葡萄糖电极与我们常规方法的结果之比低于校准液与全血中水浓度之间的预期比值，该预期比值为1.18。取决于扩散系数和血细胞比容，从血样到葡萄糖电极的陡峭梯度可能解释了这种差异。
