Moghal Nadeem, Garcia L Rene, Khan Liakot A, Iwasaki Kouichi, Sternberg Paul W
Howard Hughes Medical Institute and Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Development. 2003 Oct;130(19):4553-66. doi: 10.1242/dev.00670.
The extent to which excitable cells and behavior modulate animal development has not been examined in detail. Here, we demonstrate the existence of a novel pathway for promoting vulval fates in C. elegans that involves activation of the heterotrimeric Galphaq protein, EGL-30. EGL-30 acts with muscle-expressed EGL-19 L-type voltage-gated calcium channels to promote vulva development, and acts downstream or parallel to LET-60 (RAS). This pathway is not essential for vulval induction on standard Petri plates, but can be stimulated by expression of activated EGL-30 in neurons, or by an EGL-30-dependent change in behavior that occurs in a liquid environment. Our results indicate that excitable cells and animal behavior can provide modulatory inputs into the effects of growth factor signaling on cell fates, and suggest that communication between these cell populations is important for normal development to occur under certain environmental conditions.
可兴奋细胞和行为在多大程度上调节动物发育尚未得到详细研究。在这里，我们证明了秀丽隐杆线虫中存在一种促进外阴命运的新途径，该途径涉及异源三聚体Gαq蛋白EGL-30的激活。EGL-30与肌肉表达的EGL-19 L型电压门控钙通道共同作用以促进外阴发育，并在LET-60（RAS）的下游或与之平行发挥作用。该途径在标准培养皿上对外阴诱导并非必不可少，但可通过神经元中活化EGL-30的表达或液体环境中发生的EGL-30依赖性行为变化来刺激。我们的结果表明，可兴奋细胞和动物行为可以为生长因子信号对细胞命运的影响提供调节性输入，并表明这些细胞群体之间的通讯对于在某些环境条件下正常发育的发生很重要。
