Yoo Andrew S, Bais Carlos, Greenwald Iva
Integrated Program in Cellular, Molecular, and Biophysical Studies, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, Room 720, New York, NY 10032, USA.
Science. 2004 Jan 30;303(5658):663-6. doi: 10.1126/science.1091639.
The Caenorhabditis elegans vulva is an important paradigm for cell-cell interactions in animal development. The fates of six vulval precursor cells are patterned through the action of the epidermal growth factor receptor-mitogen-activated protein kinase (EGFR-MAPK) inductive signaling pathway, which specifies the 1 degrees fate, and the LIN-12/Notch lateral signaling pathway, which specifies the 2 degrees fate. Here, we provide evidence that the inductive signal is spatially graded and initially activates the EGFR-MAPK pathway in the prospective 2 degrees cells. Subsequently, this effect is counteracted by the expression of multiple new negative regulators of the EGFR-MAPK pathway, under direct transcriptional control of the LIN-12-mediated lateral signal.
秀丽隐杆线虫的阴门是动物发育中细胞间相互作用的重要范例。六个阴门前体细胞的命运通过表皮生长因子受体-丝裂原活化蛋白激酶（EGFR-MAPK）诱导信号通路的作用进行模式化，该通路决定1°命运，以及LIN-12/Notch侧向信号通路，该通路决定2°命运。在这里，我们提供证据表明，诱导信号在空间上是分级的，并且最初在前体2°细胞中激活EGFR-MAPK通路。随后，在LIN-12介导的侧向信号的直接转录控制下，EGFR-MAPK通路的多个新的负调节因子的表达抵消了这种作用。
