来自紫色链霉菌的阿克拉霉素-10-甲酯酶和阿克拉霉素-10-羟化酶的结晶及初步X射线衍射研究。
Crystallization and preliminary X-ray diffraction studies of aclacinomycin-10-methyl esterase and aclacinomycin-10-hydroxylase from Streptomyces purpurascens.
作者信息
Jansson Anna, Niemi Jarmo, Mäntsälä Pekka, Schneider Gunter
机构信息
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-17 77 Stockholm, Sweden.
出版信息
Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1637-9. doi: 10.1107/s0907444903014100. Epub 2003 Aug 19.
Two enzymes participating in the biosynthesis of anthracyclines in Streptomyces purpurascens, aclacinomycin-10-methyl esterase (RdmC) and aclacinomycin-10-hydroxylase (RdmB), have been crystallized. RdmB is a S-adenosyl-methionine-dependent hydroxylase and RdmC hydrolyses the carboxymethyl group of the aglycone skeleton of aclacinomycin. Crystals of RdmB obtained in the presence of S-adenosyl-L-methionine were orthorhombic, space group C222(1), with unit-cell parameters a = 63.2, b = 92.2, c = 115.3 A; diffraction data were collected to 2.1 A resolution. RdmC was crystallized as a complex with the substrate, aclacinomycin T. These crystals diffracted to 1.45 A resolution and belonged to space group P2(1), with unit-cell parameters a = 38.2, b = 84.7, c = 44.3 A, beta = 99.9 degrees.
参与紫色链霉菌中蒽环类抗生素生物合成的两种酶，阿克拉霉素-10-甲酯酶（RdmC）和阿克拉霉素-10-羟化酶（RdmB）已被结晶。RdmB是一种依赖S-腺苷甲硫氨酸的羟化酶，RdmC水解阿克拉霉素糖苷配基骨架的羧甲基。在S-腺苷-L-甲硫氨酸存在下获得的RdmB晶体为正交晶系，空间群C222(1)，晶胞参数a = 63.2，b = 92.2，c = 115.3 Å；衍射数据收集至2.1 Å分辨率。RdmC与底物阿克拉霉素T形成复合物结晶。这些晶体衍射至1.45 Å分辨率，属于空间群P2(1)，晶胞参数a = 38.2，b = 84.7，c = 44.3 Å，β = 99.9°。
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