来自利尻链霉菌的新型S-腺苷-L-甲硫氨酸依赖性C-甲基转移酶CouO的结晶及初步衍射数据分析。
Crystallization of the novel S-adenosyl-L-methionine-dependent C-methyltransferase CouO from Streptomyces rishiriensis and preliminary diffraction data analysis.
作者信息
Lyskowski Andrzej, Tengg Martin, Steinkellner Georg, Schwab Helmut, Gruber-Khadjawi Mandana, Gruber Karl
机构信息
AAustrian Centre of Industrial Biotechnology, GmbH, Petersgasse 14, 8010 Graz, Austria.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):698-700. doi: 10.1107/S1744309112017137. Epub 2012 May 23.
Abstract
Recombinant Q9F8T9 protein from Streptomyces rishiriensis (CouO), an S-adenosyl-L-methionine-dependent C-methyltransferase, has been successfully cloned, expressed and purified. CouO was crystallized from a single condition in the Morpheus crystallization screen. A vitrified crystal diffracted to 2.05 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 33.02, b = 82.87, c = 76.77 Å, β = 96.93°.
摘要
来自立山链霉菌（CouO）的重组Q9F8T9蛋白是一种依赖S-腺苷-L-甲硫氨酸的C-甲基转移酶，已成功克隆、表达和纯化。CouO在Morpheus结晶筛选的单一条件下结晶。一个玻璃化晶体的衍射分辨率为2.05 Å，属于空间群P2(1)，晶胞参数为a = 33.02、b = 82.87、c = 76.77 Å，β = 96.93°。
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