谷氨酸棒杆菌磷酸烯醇丙酮酸羧激酶的结晶及初步X射线晶体学研究

Crystallization and preliminary X-ray crystallographic studies of phosphoenolpyruvate carboxykinase from Corynebacterium glutamicum.

作者信息

Aich Sanjukta, Imabayashi Fumie, Delbaere Louis T J

机构信息

Department of Biochemistry, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan S7N 5E5, Canada.

出版信息

Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1640-1. doi: 10.1107/s0907444903014112. Epub 2003 Aug 19.

DOI:10.1107/s0907444903014112

PMID:12925798

Abstract

Phosphoenolpyruvate carboxykinase (PCK) is a key enzyme involved in the regulation of gluconeogenesis. PCKs from higher animals require guanosine nucleotide for activity. PCK from Corynebacterium glutamicum is also GTP specific. X-ray diffraction data from a C. glutamicum PCK crystal were collected to 2.8 A resolution. The crystals were monoclinic, belonging to space group P2(1), with unit-cell parameters a = 71.7, b = 117.4, c = 161.3 A, beta = 92.9 degrees. The presence of two molecules in the crystallographic asymmetric unit gives a V(M) of 2.5 A(3) Da(-1) and a solvent content of 50.3%.

摘要

磷酸烯醇式丙酮酸羧激酶（PCK）是参与糖异生调节的关键酶。高等动物的PCK活性需要鸟苷核苷酸。谷氨酸棒杆菌的PCK也对GTP具有特异性。收集了谷氨酸棒杆菌PCK晶体的X射线衍射数据，分辨率达到2.8埃。晶体为单斜晶系，属于空间群P2(1)，晶胞参数a = 71.7，b = 117.4，c = 161.3埃，β = 92.9°。晶体学不对称单元中存在两个分子，V(M)为2.5埃³Da⁻¹，溶剂含量为50.3%。