维生素C和K3对卵巢癌的体外抗肿瘤活性。

The in vitro antitumor activity of vitamins C and K3 against ovarian carcinoma.

作者信息

von Gruenigen Vivian E, Jamison James M, Gilloteaux Jacques, Lorimer Heather E, Summers Marcia, Pollard Robert R, Gwin Carley A, Summers Jack L

机构信息

Department of Obstetrics and Gynecology, SUMMA Health System, 525 E. Market Street, Akron, OH 44309, USA.

出版信息

Anticancer Res. 2003 Jul-Aug;23(4):3279-87.

PMID:12926064

Abstract

BACKGROUND

The objective was to evaluate the cytotoxic effect and mechanism of action of vitamins C (VC) and K3 (VK3) on ovarian carcinoma.

MATERIALS AND METHODS

Cytotoxicity assays were performed on ovarian cancer cell lines with VC, VK3 or a VC/VK3 combination. FIC index was employed to evaluate synergism. Flow cytometry was accomplished at 90% cytotoxic doses. Light, transmission electron microscopy and DNA isolation were performed.

RESULTS

Antitumor activity was exhibited by both VC, VK3 and VC/VK3. VC/VK3 demonstrated synergistic activity. VC/VK3 may induce a G1 block in the cell cycle. Combined vitamin treatment resulted in cells that maintain apparently intact nuclei while extruding pieces of organelle-free cytoplasm. Degradation of chromosomal DNA was observed.

CONCLUSION

Cell death (autoschizis) displayed characteristics of both apoptosis and necrosis. The cytotoxic effects observed may enable vitamins C and K3 to play an adjuvant role in the treatment of ovarian cancer.

摘要