Sainz J, Prats E, Ruiz S, Cornudella L
Department of Molecular and Cell Biology, Centro de Investigación y Desarrollo del CSIC, Barcelona, Spain.
Biochimie. 1992 Dec;74(12):1067-74. doi: 10.1016/0300-9084(92)90004-x.
The abundance of repetitive DNA in the haploid sea cucumber genome has been determined by screening a Holothuria genomic DNA library for clones containing repeated sequences using reverse genome hybridization. Analysis by in situ plaque hybridization of a set of 1132 clones has revealed the presence of repetitive DNA sequences in about 38.1% of the clones screened. The distribution of the reiterated DNA has been further analyzed by restriction endonuclease digestion of seven randomly selected repetitive clones. The repeated sequences have a fairly uniform distribution of lengths with an average length value of 7.3 kb. Analysis of the measurements suggests that the repetitive sequences are interspersed among longer single copy sequences with an average spacing interval of about 47.3 kb indicating that the repetitive and single copy DNA in the Holothuria genome are arranged in a long-period interspersion pattern.
通过使用反向基因组杂交筛选含有重复序列的克隆，来确定单倍体海参基因组中重复DNA的丰度。对一组1132个克隆进行原位噬菌斑杂交分析，结果显示在筛选的克隆中约38.1%存在重复DNA序列。通过对7个随机选择的重复克隆进行限制性内切酶消化，进一步分析了重复DNA的分布。重复序列的长度分布相当均匀，平均长度值为7.3 kb。对测量结果的分析表明，重复序列散布在较长的单拷贝序列之间，平均间隔约为47.3 kb，这表明海参基因组中的重复DNA和单拷贝DNA以长周期散布模式排列。
