Gavrović-Jankulović M, Cirković T, Burazer L, Vucković O, Jankov R M
Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Yugoslavia.
J Investig Allergol Clin Immunol. 2002;12(4):279-86.
The presence of IgE reactivity to kiwi fruit and grass pollen allergens which could be caused by cross-reactivity has been detected in many patients with allergy. Proper identification of allergens as well as cross-reactive components is essential for understanding fruit- and pollen-associated hypersensitivity.
Using the sera from the polysensitized patients with specific IgE to grass pollen and kiwi fruit we tested reactivity to both allergen sources. IgE reactivity was exhibited in 8 serum samples by immunoblot. A serum pool formed by 8 individual sera was used for the investigation of IgE crossreactivity. SDS-PAGE immunoblot-inhibition assay was performed by preincubation of the sera with meadow fescue pollen, kiwi fruit extract, and isolated 24 kDa kiwi protein. To determine the allergens of kiwi fruit extract, we performed 2D PAGE immunoblot. In order to detect the crossreactive components between two allergen sources, a specific IgE for the 24 kDa kiwi allergen was purified.
SDS-PAGE immunoblot meadow fescue pollen showed allergens ranging from 94 to 16 kDa, and kiwi fruit had 12 allergens ranging from 94 to 17 kDa. 2D-PAGE analysis revealed at least 15 spots in the kiwi extract and about 10 allergens. The most prominent allergen in 2D PAGE immunoblot was protein with 24 kDa and pI 9.4-9.5. Using an affinity-purified specific IgE we found that the 24 kDa kiwi allergen shared IgE-reactive epitopes with the meadow fescue group 4 and allergen about 36 kDa. Crossreactivity between isolated 24 kDa kiwi allergen and Fes p 4 was confirmed by anti-grass group 4 moab 2D8.
Our findings showed that fescue meadow pollen cross-sensitize to kiwi fruits. A 24 kDa kiwi glycoprotein represent potential major allergen, which share common epitopes with Fes p 4 and 36 kDa meadow fescue allergen.
在许多过敏患者中已检测到对猕猴桃和草花粉过敏原的IgE反应性，这可能是由交叉反应引起的。正确识别过敏原以及交叉反应成分对于理解水果和花粉相关的超敏反应至关重要。
使用对草花粉和猕猴桃具有特异性IgE的多敏患者血清，我们检测了对两种过敏原来源的反应性。通过免疫印迹在8份血清样本中显示出IgE反应性。由8份个体血清形成的血清池用于研究IgE交叉反应性。通过将血清与草地羊茅花粉、猕猴桃提取物和分离的24 kDa猕猴桃蛋白预孵育，进行SDS-PAGE免疫印迹抑制试验。为了确定猕猴桃提取物的过敏原，我们进行了二维聚丙烯酰胺凝胶电泳免疫印迹。为了检测两种过敏原来源之间的交叉反应成分，纯化了针对24 kDa猕猴桃过敏原的特异性IgE。
SDS-PAGE免疫印迹显示草地羊茅花粉的过敏原范围为94至16 kDa，猕猴桃有12种过敏原，范围为94至17 kDa。二维聚丙烯酰胺凝胶电泳分析显示猕猴桃提取物中至少有15个斑点和约10种过敏原。二维聚丙烯酰胺凝胶电泳免疫印迹中最突出的过敏原是分子量为24 kDa、等电点为9.4 - 9.5的蛋白质。使用亲和纯化的特异性IgE，我们发现24 kDa猕猴桃过敏原与草地羊茅第4组和约36 kDa的过敏原具有IgE反应性表位。抗草第4组单克隆抗体2D8证实了分离的24 kDa猕猴桃过敏原与Fes p 4之间的交叉反应性。
我们的研究结果表明，草地羊茅花粉与猕猴桃存在交叉致敏。一种24 kDa的猕猴桃糖蛋白代表潜在的主要过敏原，它与Fes p 4和36 kDa草地羊茅过敏原具有共同表位。
