Moore S S, Li C, Basarab J, Snelling W M, Kneeland J, Murdoch B, Hansen C, Benkel B
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
J Anim Sci. 2003 Aug;81(8):1919-25. doi: 10.2527/2003.8181919x.
Backfat thickness is one of the major quantitative traits that affect carcass quality in beef cattle. In this study, we have fine mapped a QTL for backfat EBV on bovine chromosome 14, using an identical-by-descent haplotype-sharing analysis, in a commercial line of Bos taurus. We also examined the association between gene-specific single nucleotide polymorphism (SNP) markers of the genes diacylgcerol acyltransferase 1 (DGAT1) and thyroglobulin (TG) and the backfat EBV. The results indicate that the QTL region for backfat identified on chromosome 14 is in agreement with previous studies. However, neither of the two polymorphisms of candidate genes tested, DGAT1 nor TG, showed a significant (P > 0.10) association with the backfat EBV in the cattle populations examined. However, a strong association (P = 0.0058) was detected between a microsatellite marker (CSSM66) lying approximately mid-way between the two candidate genes and the backfat EBV. These results suggest that other SNP of DGAT1, TG, or other gene(s) in the chromosomal region should be examined to test whether they have a significant effect on lipid metabolism.
背膘厚度是影响肉牛胴体品质的主要数量性状之一。在本研究中，我们利用同宗相同单倍型共享分析，在一个商业品系的黄牛中对牛14号染色体上的背膘估计育种值（EBV）的一个数量性状基因座（QTL）进行了精细定位。我们还研究了二酰基甘油酰基转移酶1（DGAT1）和甲状腺球蛋白（TG）基因的基因特异性单核苷酸多态性（SNP）标记与背膘EBV之间的关联。结果表明，在14号染色体上鉴定出的背膘QTL区域与先前的研究一致。然而，在所检测的牛群体中，候选基因DGAT1和TG的两种多态性均未显示出与背膘EBV有显著（P>0.10）关联。然而，在两个候选基因之间大约中间位置的一个微卫星标记（CSSM66）与背膘EBV之间检测到强关联（P = 0.0058）。这些结果表明，应该检测DGAT1、TG或该染色体区域中其他基因（们）的其他SNP，以测试它们是否对脂质代谢有显著影响
