Kneeland J, Li C, Basarab J, Snelling W M, Benkel B, Murdoch B, Hansen C, Moore S S
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
J Anim Sci. 2004 Dec;82(12):3405-14. doi: 10.2527/2004.82123405x.
We report the identification and fine mapping of QTL for birth weight (BWT), preweaning ADG (PWADG), and postweaning ADG on feed (ADGF) in a commercial line of Bos taurus using an identical-by-descent haplotype sharing method. One hundred seventy-six calves of 12 bulls (9 to 30 male calves from each sire) of the Beefbooster, Inc., M1 line were typed using 71 genetic markers from bovine chromosomes (BTA) 2, 6, 14, 19, 21, and 23 (8 to 16 markers from each chromosome). Sixteen haplotypes were found to have significant (P <0.05) associations with BWT at the comparison-wise threshold. The 16 haplotypes span 13 chromosomal regions, two on BTA 2 (9.1 to 22.5 cM and 95.0 to 100.3 cM), three on BTA 6 (8.2 to 11.8 cM, 35.5 to 49.7 cM, and 83.0 to 86.2 cM), three on BTA 14 (26.0 to 26.7 cM, 36.2 to 46.2 cM, and 52.0 to 67.7 cM), one on BTA 19 (52.0 to 52.7 cM), two on BTA 21 (9.9 to 20.4 cM and 28.2 to 46.1 cM), and two on BTA 23 (23.9 to 36.0 cM and 45.1 to 50.9 cM). Thirteen haplotypes spanning seven chromosomal regions significantly affected (P <0.05) PWADG at the comparison-wise threshold. The seven chromosomal regions include two regions on BTA 6 (11.8 to 44.2 cM and 83.0 to 86.2 cM), one on BTA 14 (26.7 to 50.8 cM), one on BTA 19 (4.8 to 15.9 cM), one on BTA 21 (9.9 to 20.4 cM), and two on BTA 23 (17.3 to 36.0 cM and 45.1 to 50.9 cM). For ADGF, 11 haplotypes were identified to have significant associations (P <0.05) at the comparison-wise threshold. The 11 haplotypes represented eight chromosomal regions, one on BTA 2 (9.1 to 22.5 cM), two on BTA 6 (49.7 to 50.1 cM and 59.6 to 63.6 cM), two on BTA 14 (17.0 to 24.0 cM and 36.2 to 46.2 cM), two on BTA 19 (52.0 to 52.7 cM and 65.1 to 65.7 cM), and one on BTA 21 (46.1 to 53.1 cM). The QTL regions identified and fine mapped in this study will provide a reference for future positional candidate gene research and marker-assisted selection of various growth traits.
我们报告了使用同源单倍型共享方法，对商业肉牛品系中出生体重（BWT）、断奶前平均日增重（PWADG）和断奶后采食平均日增重（ADGF）的数量性状基因座（QTL）进行的鉴定和精细定位。对Beefbooster公司M1品系的12头公牛的176头犊牛（每个父本有9至30头雄性犊牛），使用来自牛染色体（BTA）2、6、14、19、21和23的71个遗传标记进行分型（每条染色体有8至16个标记）。在比较水平阈值下，发现16个单倍型与BWT有显著关联（P <0.05）。这16个单倍型跨越13个染色体区域，BTA 2上有两个区域（9.1至22.5厘摩和95.0至100.3厘摩），BTA 6上有三个区域（8.2至11.8厘摩、35.5至49.7厘摩和83.0至86.2厘摩），BTA 14上有三个区域（26.0至26.7厘摩、36.2至46.2厘摩和52.0至67.7厘摩），BTA 19上有一个区域（52.0至52.7厘摩），BTA 21上有两个区域（9.9至20.4厘摩和28.2至46.1厘摩），BTA 23上有两个区域（23.9至36.0厘摩和45.1至50.9厘摩）。在比较水平阈值下，13个跨越7个染色体区域的单倍型对PWADG有显著影响（P <0.05）。这7个染色体区域包括BTA 6上的两个区域（11.8至44.2厘摩和83.0至86.2厘摩），BTA 14上的一个区域（26.7至50.8厘摩），BTA 19上的一个区域（4.8至15.9厘摩），BTA 21上的一个区域（9.9至20.4厘摩），以及BTA 23上的两个区域（17.3至36.0厘摩和45.1至50.9厘摩）。对于ADGF，在比较水平阈值下鉴定出11个单倍型有显著关联（P <0.05）。这11个单倍型代表8个染色体区域，BTA 2上有一个区域（9.1至22.5厘摩），BTA 6上有两个区域（49.7至50.1厘摩和59.6至63.6厘摩），BTA 14上有两个区域（17.0至24.0厘摩和36.2至46.2厘摩），BTA 19上有两个区域（52.0至52.7厘摩和65.1至65.7厘摩），BTA 21上有一个区域（46.1至53.1厘摩）。本研究中鉴定和精细定位的QTL区域将为未来各种生长性状的位置候选基因研究和标记辅助选择提供参考。
