Yokoi H, Seki M, Okazoe S, Okumura K, Yoshikawa H
Department of Otorhinolaryngology, Juntendo University School of Medicine, Tokyo, Japan.
Lymphology. 2003 Jun;36(2):74-83.
In this study, we investigated the expression and function of CD27 and CD70 in the human tonsil. We found that IgG production was significantly reduced after the administration of anti-CD27 and anti-CD70 antibodies in the T cell dependent B cell activation system, suggesting the involvement of CD27/CD70 in the production of IgG. In addition, the amounts of peripheral and tonsililar CD4+ CD27+ cells were almost identical, whereas the expression of CD4+ CD27- cells was higher (4.9%) in tonsil than in peripheral blood (2.4%). Furthermore, anti-CD27 antibody and anti-CD4 antibody-positive cells were found mainly in the interfollicular region, and a small number of this cell population was observed in both germinal center (GC) and mantle. Double-staining showed that the germinal center was almost completely composed of CD4+ CD27+ cells. In contrast to CD27 cells, only a small number of CD70+ cells were present in the follicles. Moreover, anti-CD19 antibody, a B cell marker, was stained in the GC and mantle zone, and CD19+ CD27+ cells were observed in the GC close to the mantle. The above findings favor the involvement and role of CD27/CD70 in the T-B cell interaction by inducing IgG production and increasing memory cell (CD4+ CD27-) number in the tonsil.
在本研究中，我们调查了CD27和CD70在人扁桃体中的表达及功能。我们发现在T细胞依赖性B细胞活化系统中给予抗CD27和抗CD70抗体后，IgG产生显著减少，这表明CD27/CD70参与了IgG的产生。此外，外周血和扁桃体中CD4+ CD27+细胞的数量几乎相同，而扁桃体中CD4+ CD27-细胞的表达（4.9%）高于外周血（2.4%）。此外，抗CD27抗体和抗CD4抗体阳性细胞主要见于滤泡间区，在生发中心（GC）和套区均观察到少量该细胞群体。双重染色显示生发中心几乎完全由CD4+ CD27+细胞组成。与CD27细胞相反，滤泡中仅存在少量CD70+细胞。此外，B细胞标志物抗CD19抗体在GC和套区呈阳性染色，在靠近套区的GC中观察到CD19+ CD27+细胞。上述发现支持CD27/CD70通过诱导IgG产生和增加扁桃体中记忆细胞（CD4+ CD27-）数量而参与T-B细胞相互作用并发挥作用。
