Agematsu K, Kobata T, Yang F C, Nakazawa T, Fukushima K, Kitahara M, Mori T, Sugita K, Morimoto C, Komiyama A
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Eur J Immunol. 1995 Oct;25(10):2825-9. doi: 10.1002/eji.1830251017.
CD27 is a T cell activation antigen expressed on a majority of peripheral blood T cells. CD27 is also expressed on a subpopulation of human B cells, and it is reported that CD27+ B cells secrete both IgG and IgM. CD70, a ligand for CD27, is expressed on activated T and B cells, suggesting an interaction between T and B cells via CD27/CD70 ligation. Here, we analyze B cell immunoglobulin synthesis using a CD70 transfectant and present functional data showing that B cells secrete large amounts of IgG and IgM as a result of the CD27/CD70 interaction. A flow cytometric analysis showed that CD27 expression was increased and CD70 was expressed on tonsillar and peripheral blood B cells after activation with Staphylococcus aureus Cowan strain (SAC) plus interleukin (IL-2). In addition, the proliferation of B cells was enhanced mildly by the addition of CD70 transfectant, and its proliferation was blocked by anti-CD70 mAb. More importantly, the CD70 transfectant enhanced IgG and IgM production by purified B cells greatly in the presence of SAC plus IL-2. The enhancement was completely blocked by the addition of either anti-CD70 mAb or anti-CD27 mAb. Strongly suggesting that the interaction of CD27 with its ligand, CD70, on B cells plays an important role in B cell growth and differentiation to produce IgG and IgM.
CD27是一种在大多数外周血T细胞上表达的T细胞活化抗原。CD27也在人类B细胞的一个亚群上表达，据报道CD27+ B细胞分泌IgG和IgM。CD70是CD27的配体，在活化的T细胞和B细胞上表达，提示T细胞和B细胞之间通过CD27/CD70连接发生相互作用。在此，我们使用CD70转染细胞分析B细胞免疫球蛋白的合成，并呈现功能数据表明，由于CD27/CD70相互作用，B细胞分泌大量的IgG和IgM。流式细胞术分析显示，在用金黄色葡萄球菌考恩菌株（SAC）加白细胞介素（IL-2）激活后，扁桃体和外周血B细胞上的CD27表达增加且有CD70表达。此外，添加CD70转染细胞可轻度增强B细胞的增殖，而其增殖被抗CD70单克隆抗体阻断。更重要的是，在SAC加IL-2存在的情况下，CD70转染细胞极大地增强了纯化B细胞的IgG和IgM产生。添加抗CD70单克隆抗体或抗CD27单克隆抗体可完全阻断这种增强作用。强烈提示CD27与其配体CD70在B细胞上的相互作用在B细胞生长和分化以产生IgG和IgM中起重要作用。
