Taïbi Faïza, Smagghe Guy, Amrani Leïla, Soltani-Mazouni Nadia
Laboratoire de Biologie Animale Appliquée, Université d'Annaba, 23000 Annaba, Algeria.
Comp Biochem Physiol C Toxicol Pharmacol. 2003 Jul;135C(3):257-67. doi: 10.1016/s1532-0456(03)00112-1.
RH-0345 belongs to a new group of insect growth regulators (IGRs) with a benzoylhydrazine structure that mimic the action of the natural insect molting hormone 20-hydroxyecdysone. After topical application on female adult beetles of mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae), first oviposition was delayed, the number of eggs per female was reduced by 32%, the follicular epithelium was thinner (-33%) during sexual maturation, the size of deposited eggs was reduced, and egg viability was lost by 68%. Treatment with RH-0345 had also reduced the ovarian protein content and two protein bands were missing in the ovaries. Ultrastructural observations of the ovaries at the end of vitellogenesis in treated females, however, showed no evident differences with the fine structure of both follicular cells and oocytes in controls. In addition, we measured the amount of ecdysteroids in the medium of treated ovary cultures in vitro and in the eggs deposited by treated females. Possible action sites with the reproductive system at different levels in T. molitor are discussed for this novel group of IGRs.
RH - 0345属于一类新型的昆虫生长调节剂（IGRs），具有苯甲酰肼结构，可模拟天然昆虫蜕皮激素20 - 羟基蜕皮酮的作用。将其局部施用于黄粉虫成虫雌性（黄粉虫，鞘翅目拟步甲科）后，首次产卵延迟，每只雌性的产卵数减少32%，性成熟期间卵泡上皮变薄（-33%），所产卵子大小减小，且卵子活力丧失68%。用RH - 0345处理还降低了卵巢蛋白质含量，卵巢中两条蛋白带缺失。然而，对处理后雌性卵黄发生末期卵巢的超微结构观察显示，卵泡细胞和卵母细胞的精细结构与对照相比无明显差异。此外，我们测量了体外处理的卵巢培养物培养基以及处理后雌性所产卵子中的蜕皮甾体含量。针对这类新型IGRs，讨论了其在黄粉虫不同水平生殖系统上可能的作用位点。
