Bagis S, Comelekoglu U, Coskun B, Milcan A, Buyukakilli B, Sahin G, Ozisik S, Erdogan C
Mersin University, Adana, Turkey.
Lasers Med Sci. 2003;18(2):83-8. doi: 10.1007/s10103-003-0258-6.
We evaluated the electrophysiological and histopathological effects of low-energy gallium arsenide (904 nm) laser irradiation on the intact skin injured rat sciatic nerve. Twenty-four male Wistar rats were divided into three groups ( n=8 each). At the level of proximal third of the femur the sciatic nerve was crushed bilaterally with an aneurysm clip (Aesculap FE 751, Tuttingen, Germany) for half a second. A gallium arsenide laser (wavelength 904 nm, pulse duration 220 ns, peak power per pulse 27 W, spot size 0.28 cm2, pulse repetition rate 16, 128 and 1000 Hz; total applied energy density 0.31, 2.48 and 19 J/cm2) was applied to the right sciatic nerve for 15 min daily at the same time on 7 consecutive days. The same procedure was performed on the left sciatic nerve of same animal, but without radiation emission, and this was accepted as control. Compound muscle action potentials were recorded from right and left sides in all three groups before surgery, just at the end of injury, at the 24th hour and on the 14th and 21st days of injury in all rats using a BIOPAC MP 100 Acquisition System Version 3.5.7 (Santa Barbara, USA). BIOPAC Acknowledge Analysis Software (ACK 100 W) was used to measure CMAP amplitude, area, proximal and distal latency, total duration and conduction velocity. Twenty-one days after injury, the rats were sacrificed. The sciatic nerves of the operated parts were harvested from the right and left sides. Histopathological evaluation was performed by light microscopy. Statistical evaluation was done using analysis of variance for two factors (right and left sides) repeated-measures (CMAP variables within groups) and the Tukey-Kramer Honestly Significant Difference test (CMAP variables between laser groups). The significance was set at p < 0.05. No statistically significant difference (p > 0.05) was found regarding the amplitude, area, duration and conduction velocity of CMAP for each applied dose (0.31, 2.48 and 19 J/cm2) on the irradiated (right) side and the control (left) side, or between irradiated groups. Twenty-one days after injury there were no qualitative differences in the morphological pattern of the regenerated nerve fibres in either irradiated (0.31, 2.48 and 19 J/cm2) or control nerves when evaluated by light microscopy. This study showed that low-energy GaAs irradiation did not have any effect on the injured rat sciatic nerve.
我们评估了低能量砷化镓（904纳米）激光照射对完整皮肤损伤的大鼠坐骨神经的电生理和组织病理学影响。将24只雄性Wistar大鼠分为三组（每组n = 8）。在股骨近端三分之一水平，用动脉瘤夹（德国图廷根蛇牌FE 751）双侧夹闭坐骨神经半秒钟。使用砷化镓激光（波长904纳米，脉冲持续时间220纳秒，每个脉冲的峰值功率27瓦，光斑尺寸0.28平方厘米，脉冲重复频率16、128和1000赫兹；总施加能量密度0.31、2.48和19焦/平方厘米），连续7天每天同时对右侧坐骨神经照射15分钟。对同一动物的左侧坐骨神经进行相同操作，但不发射激光，将其作为对照。在所有大鼠手术前、损伤刚结束时、损伤后第24小时以及损伤后第14天和第21天，使用美国加利福尼亚州圣巴巴拉市BIOPAC公司的MP 100采集系统3.5.7版，从右侧和左侧记录复合肌肉动作电位。使用BIOPAC Acknowledge分析软件（ACK 100 W）测量复合肌肉动作电位的波幅、面积、近端和远端潜伏期、总持续时间和传导速度。损伤21天后，处死大鼠。从右侧和左侧取出手术部位的坐骨神经。通过光学显微镜进行组织病理学评估。采用双因素（右侧和左侧）重复测量方差分析（组内复合肌肉动作电位变量）和Tukey-Kramer诚实显著差异检验（激光组间复合肌肉动作电位变量）进行统计学评估。显著性设定为p < 0.05。对于每个施加剂量（0.31、2.48和19焦/平方厘米），照射侧（右侧）和对照侧（左侧）的复合肌肉动作电位的波幅、面积、持续时间和传导速度，或照射组之间，均未发现统计学显著差异（p > 0.05）。损伤21天后，通过光学显微镜评估，照射（0.31、2.48和19焦/平方厘米）或对照神经中再生神经纤维的形态模式没有定性差异。本研究表明，低能量砷化镓激光照射对损伤的大鼠坐骨神经没有任何影响。
