Ito Masako, Ejiri Sadakazu, Jinnai Hiroshi, Kono Jun, Ikeda Satoshi, Nishida Akifumi, Uesugi Kentaro, Yagi Naoto, Tanaka Mikako, Hayashi Kuniaki
Department of Radiology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, 852-8501 Nagasaki, Japan.
J Bone Miner Metab. 2003;21(5):287-93. doi: 10.1007/s00774-003-0422-x.
We investigated the ability of synchrotron radiation computed tomography (SR-CT) to demonstrate trabecular microstructure, detail of trabecular surfaces, and mineralization of bones. Eight rat vertebrae, six rat tibiae, and eight minipig vertebrae were scanned using SR-CT at the synchrotron radiation facility Super Photon ring-8GeV (SPring-8). Images obtained using conventional micro-CT, scanning electron microscopy (SEM), and contact microradiography (CMR) were compared with the SR-CT images. SR-CT showed high image quality without visible partial volume effect. Three-dimensional SR-CT revealed shallow concavities in the bone surface, which were considered to correspond to osteoclastic resorption areas, as well as the connectivity, anisotropy, and shape (rod- or platelike) of trabeculae. Two-dimensional SR-CT showed different density along the surface of the trabecular bone, indicating the degree of bone mineralization. In conclusion, SR-CT seems to be a useful tool for delineating trabecular surfaces, evaluating bone mineralization, and revealing precise trabecular structure.
我们研究了同步辐射计算机断层扫描（SR-CT）显示骨小梁微观结构、骨小梁表面细节以及骨矿化情况的能力。使用8GeV超级光子环（SPring-8）同步辐射设施的SR-CT对8个大鼠椎骨、6个大鼠胫骨和8个小型猪椎骨进行了扫描。将使用传统显微CT、扫描电子显微镜（SEM）和接触式显微放射摄影（CMR）获得的图像与SR-CT图像进行了比较。SR-CT显示出高图像质量，没有明显的部分容积效应。三维SR-CT揭示了骨表面的浅凹，这些浅凹被认为对应于破骨细胞吸收区域，以及骨小梁的连通性、各向异性和形状（杆状或板状）。二维SR-CT显示沿骨小梁表面有不同的密度，表明骨矿化程度。总之，SR-CT似乎是描绘骨小梁表面、评估骨矿化和揭示精确骨小梁结构的有用工具。
