检测肌酸激酶同工酶作为横纹肌肉瘤的肿瘤标志物

Detection of creatine kinase isoenzymes as tumoral markers of rhabdomyosarcoma.

作者信息

Fernández J E, Rodríguez F, Recio M A, Vélez C, Aránega A E, Alvarez L, Aránega A

机构信息

Department of Morphological Sciences, School of Medicine, University of Granada, Spain.

出版信息

Enzyme. 1992;46(4-5):245-8. doi: 10.1159/000468795.

DOI:10.1159/000468795

PMID:1292934

Abstract

We analyzed the expression profile of isoenzymatic fractions of creatine phosphokinase (EC 2.7.3.2) isotypes MM, MB and BB in three cell lines derived from embryonic rhabdomyosarcomas and a normal counterpart cell line. Electrophoretic data showed that the BB fraction was consistently expressed de novo, in contrast with its counterpart in normal tissue. The BB fraction may serve as new tumoral marker for the diagnosis of rhabdomyosarcoma. In addition, the appearance of macrocreatine kinase type-1 in this type of neoplasm may serve to reinforce the diagnosis when rhabdomyosarcoma is suspected.

摘要

我们分析了源自胚胎横纹肌肉瘤的三种细胞系及一种正常对照细胞系中肌酸磷酸激酶（EC 2.7.3.2）同工酶MM、MB和BB亚型的表达谱。电泳数据显示，与正常组织中的对应物相比，BB亚型始终是从头表达的。BB亚型可作为横纹肌肉瘤诊断的新肿瘤标志物。此外，在这种肿瘤类型中出现的1型巨肌酸激酶，在怀疑为横纹肌肉瘤时可能有助于加强诊断。